Evidence map›Paper›PMID 40491003›Full record

ArticleBiotechnology journal2025

Simultaneous Repression of GLUCAN WATER DIKINASE 1 and STARCH BRANCHING ENZYME 1 in Potato Tubers Leads to Starch With Increased Amylose and Novel Industrial Properties.

Muyiwa S Adegbaju, Nina Gouws, Christell van der Vyver, Pedri Claassens, Jens Kossmann, Michaela Fischer-Stettler, Samuel C Zeeman, James R Lloyd

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Article in Biotechnology journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Muyiwa S AdegbajuInstitute for Plant Biotechnology, Department of Genetics, Stellenbosch University, Stellenbosch, South Africa.
Nina GouwsInstitute for Plant Biotechnology, Department of Genetics, Stellenbosch University, Stellenbosch, South Africa.
Christell van der VyverInstitute for Plant Biotechnology, Department of Genetics, Stellenbosch University, Stellenbosch, South Africa.
Pedri ClaassensInstitute for Plant Biotechnology, Department of Genetics, Stellenbosch University, Stellenbosch, South Africa.
Jens KossmannInstitute for Plant Biotechnology, Department of Genetics, Stellenbosch University, Stellenbosch, South Africa.
Michaela Fischer-StettlerInstitute of Molecular Plant Biology, ETH Zürich, Zürich, Switzerland.
Samuel C ZeemanInstitute of Molecular Plant Biology, ETH Zürich, Zürich, Switzerland.
James R LloydInstitute for Plant Biotechnology, Department of Genetics, Stellenbosch University, Stellenbosch, South Africa.ORCID https://orcid.org/0000-0003-3911-1819

Funding

National Research Foundation SRUG200306508359
6 · The paper itself

Abstract

This study examines how post-transcriptional gene silencing of STARCH BRANCHING ENZYME 1 (SBE1) and GLUCAN WATER DIKINASE 1 (GWD1) affects the structure and properties of potato tuber starch. Silencing of either gene individually or simultaneously altered starch chemistry physical properties. Repression of StGWD1 reduced phosphate content, while repression of StSBE1 increased it. The phosphate content of starch isolated from plants where both genes were repressed was increased compared to StGWD1 repressed lines, but lower than both the SBE1 repressed lines and the untransformed control. Constituent chain lengths of starches from all lines were altered, and amylose content was increased in the gwd1 and sbe1/gwd1 double repressed lines, which also accumulated small numbers of lobed starch granules. Pasting properties were also affected, with starch from StSBE1-repressed lines demonstrating increased peak and trough viscosities and gwd1 lines showing decreased peak and trough viscosities, compared with the control. Peak and trough viscosities were lowest in the sbe1/gwd1 repressed lines. We believe that these data demonstrate that alterations in starch phosphate influence the degree of branching within starch and offer a novel in planta strategy for optimizing the industrial properties of potato storage starch.

Indexed as

1,4-alpha-Glucan Branching EnzymeAmylosePhosphotransferases (Paired Acceptors)Plant ProteinsPlant TubersSolanum tuberosumStarchGene SilencingPlants, Genetically Modified1,4-alpha-Glucan Branching EnzymeAmylosePhosphotransferases (Paired Acceptors)Plant ProteinsStarchGlucan water dikinaseStarch branching enzymeStarch structure

Identifiers

PMID40491003
PMCPMC12149484

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.