Evidence map›Paper›PMID 36721204›Full record

ArticleBMC biotechnology2023

Biochemical characterization and insights into the potency of the acidic Aspergillus niger NRC114 purified α-galactosidase in removing raffinose family oligosaccharides from soymilk yogurt.

Abdelmageed M Othman, Ali M Elshafei, Maysa A Elsayed, Gamil E Ibrahim, Mohamed M Hassan, Nayra S Mehanna

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC biotechnology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
2.1field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 8 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Abdelmageed M OthmanMicrobial Chemistry Department, Biotechnology Research Institute, National Research Centre, 33 El Bohouth St., Dokki, 12622, Giza, Egypt. othmanam_nrc@yahoo.com.
Ali M ElshafeiMicrobial Chemistry Department, Biotechnology Research Institute, National Research Centre, 33 El Bohouth St., Dokki, 12622, Giza, Egypt.
Maysa A ElsayedMicrobial Chemistry Department, Biotechnology Research Institute, National Research Centre, 33 El Bohouth St., Dokki, 12622, Giza, Egypt.
Gamil E IbrahimChemistry of Flavor and Aroma Department, Food Industries and Nutrition Research Institute, National Research Centre, 33 El Bohouth St., Dokki, 12622, Giza, Egypt.
Mohamed M HassanMicrobial Chemistry Department, Biotechnology Research Institute, National Research Centre, 33 El Bohouth St., Dokki, 12622, Giza, Egypt.
Nayra S MehannaDairy Sciences Department, Food Industries and Nutrition Research Institute, National Research Centre, 33 El Bohouth St., Dokki, 12622, Giza, Egypt.
National Research Centre · EG

Funding

National Research Centre 12020114
6 · The paper itself

Abstract

backgroundBecause humans lack α-galactosidase, foods containing certain oligosaccharides from the raffinose family, such as soybeans and other legumes, may disrupt digestion and cause flatulence.

resultsAspergillus niger NRC114 α-galactosidase was purified using protein precipitation, gel filtration, and ion exchange chromatography steps, which resulted in a 123-fold purification. The purified enzyme was found to be 64 kDa using the SDS-PAGE approach. The optimum pH and temperature of the purified α-galactosidase were detected at pH 3.5 and 60 ºC, respectively. The pure enzyme exhibited potent acidic pH stability at pH 3.0 and pH 4.0 for 2 h, and it retained its full activity at 50 ºC and 60 ºC for 120 min and 90 min, respectively. The enzyme was activated using 2.5 mM of K

conclusionThe findings of this study indicate the importance of A. niger NRC114 α-galactosidase enzyme for future studies, especially its applications in a variety of biological fields.

Indexed as

alpha-GalactosidaseAspergillus nigerAspergillusHumansOligosaccharidesRaffinoseSoy FoodsYogurtalpha-GalactosidaseOligosaccharidesRaffinoseNutritional propertiesPurificationSoy beanTreated-soymilk yogurtα-galactosidase

Identifiers

PMID36721204
PMCPMC9887927
OpenAlexW4318617531

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.