ArticlePloS one2023
Thermo-neutrophilic cellulases and chitinases characterized from a novel putative antifungal biocontrol agent: Bacillus subtilis TD11.
Article in PloS one, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 26 citations in OpenAlex.
- Serial Knockout Isolation Technique (SKIT): targeted isolation of multi-antagonist bacteria.MethodsX · 2026Article
- Bacilli the most important biocontrol agent in Brazilian agriculture: from mechanisms of action to market adoption.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026Review
- The genomic resource of Lysinibacillus fusiformis KBD-5, a biocontrol agent with antifungal activity against Botrytis cinerea.BMC genomics · 2026Article
- Genophenotypic insights into biocontrol and growth-promoting mechanisms of Bacillus velezensis BV25 against tomato Fusarium wilt.BMC microbiology · 2026Article
- Fungal endophytes associated with Inca nut plants (Frontiers in microbiology · 2026Article
- Efficacy of chitinases from mangrove wetland derivedFrontiers in microbiology · 2026Article
- Identification and Biocontrol ofJournal of fungi (Basel, Switzerland) · 2025Article
- Characterization of probiotic strains of Bacillus sp. from fermented palm wine (Nypa fructicans sp.) and exploration of cellulolytic potential for use as an addition in animal feed.International microbiology : the official journal of the Spanish Society for Microbiology · 2025Article
- Genomic Functional Analysis and Cellulase Characterization for the Enzyme-Producing StrainMicroorganisms · 2025Article
- Citric Acid by-Product Fermentation byVeterinary sciences · 2024Article
- Agro Active Potential ofMicroorganisms · 2024Article
- Ecological versatility and biotechnological promise: Comprehensive characterization of the isolated thermophilic Bacillus strains.PloS one · 2024Article
- Involvement of theInternational journal of microbiology · 2024Article
- Genome and Transcriptome Analysis to Elucidate the Biocontrol Mechanism ofMicroorganisms · 2023Article
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cellulose and chitin are the most abundant naturally occurring biopolymers synthesized in plants and animals and are used for synthesis of different organic compounds and acids in the industry. Therefore, cellulases and chitinases are important for their multiple uses in industry and biotechnology. Moreover, chitinases have a role in the biological control of phytopathogens. A bacterial strain Bacillus subtilis TD11 was previously isolated and characterized as a putative biocontrol agent owing to its significant antifungal potential. In this study, cellulase and chitinase produced by the strain B. subtilis TD11 were purified and characterized. The activity of the cellulases and chitinases were optimized at different pH (2 to 10) and temperatures (20 to 90°C). The substrate specificity of cellulases was evaluated using different substances including carboxymethyl cellulose (CMC), hydroxyethyl cellulose (HEC), and crystalline substrates. The cellulase produced by B. subtilis TD11 had a molecular mass of 45 kDa while that of chitinase was 55 kDa. The optimal activities of the enzymes were found at neutral pH (6.0 to 7.0). The optimum temperature for the purified cellulases was in the range of 50 to 70°C while, purified chitinases were optimally active at 50°C. The highest substrate specificity of the purified cellulase was found for CMC (100%) followed by HEC (>50% activity) while no hydrolysis was observed against the crystalline substrates. Moreover, it was observed that the purified chitinase was inhibitory against the fungi containing chitin in their hyphal walls i.e., Rhizoctonia, Colletotrichum, Aspergillus and Fusarium having a dose-effect relationship.
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