ArticleAnimal bioscience2021
Protective effects of 5-aminolevulinic acid on heat stress in bovine mammary epithelial cells.
Article in Animal bioscience, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 18 citations in OpenAlex.
- Dietary 5-Aminolevulinic Acid Alleviates Heat Stress-Induced Renal Injury in Laying Hens by Improving Mitochondrial Quality and Enhancing Antioxidant Activity.Antioxidants (Basel, Switzerland) · 2025Article
- Transfer of Antioxidant Capacity Through Placenta and Colostrum: β-Carotene and Superoxide Dismutase Collaboratively Enhance Integrated Breeding of Sows and Piglets.Antioxidants (Basel, Switzerland) · 2025Article
- Palmitic acid-induced cell death: impact of endoplasmic reticulum and oxidative stress, mitigated by L-citrulline.Animal bioscience · 2025Article
- Effects of Dietary 5-Aminolevulinic Acid on Growth, Nutrient Composition, and Intestinal Microflora in Juvenile Shrimp, Litopenaeus vannamei.Marine biotechnology (New York, N.Y.) · 2024Article
- Article
- 5-Aminolevulinic acid combined with sodium ferrous citrate mitigates effects of heat stress on bovine oocyte developmental competence.The Journal of reproduction and development · 2022Article
- 5-Aminolevulinate improves metabolic recovery and cell survival of the liver following cold preservation.Theranostics · 2022Article
- 5-Aminolevulinic Acid Attenuates Glucose-Regulated Protein 78 Expression and Hepatocyte Lipoapoptosis via Heme Oxygenase-1 Induction.International journal of molecular sciences · 2021Article
- 5-ALA Attenuates the Palmitic Acid-Induced ER Stress and Apoptosis in Bovine Mammary Epithelial Cells.Molecules (Basel, Switzerland) · 2021Article
- Cytoprotective Effects of Taurine on Heat-Induced Bovine Mammary Epithelial Cells In Vitro.Cells · 2021Article
- Ursodeoxycholic Acid Alleviates Palmitic Acid-Induced Apoptosis in Bovine Mammary Epithelial Cells.Animal science journal = Nihon chikusan GakkaihoArticle
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveCells have increased susceptibility to activation of apoptosis when suffering heat stress (HS). An effective supplementation strategy to mimic heat-induced apoptosis of bovine mammary epithelial cells (MECs) is necessary to maintain optimal milk production. This study aimed to investigate possible protective effects of the anti-apoptotic activity of 5-aminolevulinic acid (5-ALA) against HS-induced damage of bovine MECs.
methodsBovine MECs were pretreated with or without 5-ALA at concentrations of 10, 100, and 500 μM for 24 h followed by HS (42.5°C for 24 h and 48 h). Cell viability was measured with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays. Real-time quantitative polymerase chain reaction and Western blotting were used to explore the regulation of genes associated with apoptosis, oxidative stress, and endoplasmic reticulum (ER) stress genes.
resultsWe found that 5-ALA induces cytoprotection via inhibition of apoptosis markers after HS-induced damage. Pretreatment of bovine MECs with 5-ALA resulted in dramatic upregulation of mRNA for nuclear factor erythroid-derived 2-like factor 2, heme oxygenase-1, and NAD(P)H quinone oxidoreductase 1, all of which are antioxidant stress genes. Moreover, 5-ALA pretreatment significantly suppressed HS-induced ER stress-associated markers, glucose-regulated protein 78, and C/EBP homologous protein expression levels.
conclusion5-ALA can ameliorate the ER stress in heat stressed bovine MEC via enhancing the expression of antioxidant gene.
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