ArticleMethodsX2020
Modified acid-PAGE method for rapid screening and phenotyping of wheat gliadin mutant lines.
Article in MethodsX, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
5 citing papers in PubMed, 10 citations in OpenAlex.
- Integration of HMW-GS and retrotransposon marker LTR14 for selection of wheat genotypes combining baking quality and resistance to take-all disease.BMC plant biology · 2026Article
- Development of an inexpensive, simple purification method for ω-5 gliadin from wheat flour.MethodsX · 2026Article
- Evaluation of alpha-gliadin celiac disease epitopes in some Aegilops species containing the D genome.BMC plant biology · 2026Article
- High variation in the gluten composition and grain protein content among synthetic wheat lines.PloS one · 2025Article
- Variations in the composition and frequency of celiac disease epitopes among synthetic wheat lines.Frontiers in plant science · 2024Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Acid-polyacrylamide gel electrophoresis (A-PAGE) is used to phenotype different varieties of wheat based on their gliadin profiles. The family of gliadin proteins is a major component of wheat gluten. Gluten is the major determinant of the unique viscoelastic property of wheat dough that is necessary in the production of important food products including bread, cake, cookies and pasta. However, several gliadin proteins are also known to be causal agents in triggering human immunogenic responses that lead to several gluten-related health risks like celiac disease and wheat-dependent exercise-induced anaphylaxis. Therefore, research to identify wheat lines with reduced levels of immunogenic proteins is being vigorously pursued in several laboratories around the world. Unfortunately, no commercial A-PAGE gels are currently available for cereal researchers to use for separating wheat gliadins. This work reports the development of an easy-to-use A-PAGE protocol to resolve gliadins with high reproducibility and resolution to screen and phenotype gliadin deficient lines in wheat.•This acetic acid based A-PAGE method with urea utilizes commercially available reagents and materials to make gel casting simpler and more efficient.•It can be used to phenotype different wheat varieties to establish purity and to identify mutants of wheat with altered gliadin protein profiles.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.