ArticleFoods (Basel, Switzerland)2020
A New Edible Film to Produce In Vitro Meat.
Article in Foods (Basel, Switzerland), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 55 citations in OpenAlex.
- 3D bio-screen printing for high-throughput production of scaffolds for meat alternatives.NPJ science of food · 2026Article
- Edible Scaffolds for Cultivated Meat Production.Advances in biochemical engineering/biotechnology · 2026Review
- A scaffold based on uncoated rice grains integrated with piscine cells supporting the production of a sustainable food.NPJ science of food · 2025Article
- Curcumin-incorporated edible hydrogel films based on potato starch/κ-carrageenan/poly(vinyl alcohol) for cultured meat scaffolding.Biomedical engineering online · 2025Article
- Trends in non-animal scaffolds for cultured meat structuration.NPJ science of food · 2025Review
- Beyond Imitation: How Food Colloids Are Shaping the Next Generation of Biomimetic Foods.Gels (Basel, Switzerland) · 2025Review
- Future protein alternative: recent progress and challenges in cellular agriculture.Food science and biotechnology · 2025Review
- Current challenges of alternative proteins as future foods.NPJ science of food · 2024Review
- Cell-cultivated aquatic food products: emerging production systems for seafood.Journal of biological engineering · 2024Review
- Global transcriptome profiles provide insights into muscle cell development and differentiation on microstructured marine biopolymer scaffolds for cultured meat production.Scientific reports · 2024Article
- Production of Plant-Based, Film-Type Scaffolds Using Alginate and Corn Starch for the Culture of Bovine Myoblasts.Foods (Basel, Switzerland) · 2024Article
- Utilization of Lumpfish (Marine drugs · 2024Article
- The important role of cellular mechanical microenvironment in engineering structured cultivated meat: Recent advances.Current research in food science · 2024Review
- Random cellulose acetate nanofibers: a breakthrough for cultivated meat production.Frontiers in nutrition · 2023Article
- Serum-free alginate-C2C12 cells microcapsule as a model of alternative animal protein source.Frontiers in nutrition · 2023Article
- Bioactive cellulose acetate nanofiber loaded with annatto support skeletal muscle cell attachment and proliferation.Frontiers in bioengineering and biotechnology · 2023Article
- Bioprocessing by Decellularized Scaffold Biomaterials in Cultured Meat: A Review.Bioengineering (Basel, Switzerland) · 2022Review
- Serum-free cultures of C2C12 cells show different muscle phenotypes which can be estimated by metabolic profiling.Scientific reports · 2022Article
- Scaffolding Biomaterials for 3D Cultivated Meat: Prospects and Challenges.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
- Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
In vitro meat is a novel concept of food science and biotechnology. Methods to produce in vitro meat employ muscle cells cultivated on a scaffold in a serum-free medium using a bioreactor. The microstructure of the scaffold is a key factor, because muscle cells must be oriented to generate parallel alignments of fibers. This work aimed to develop a new scaffold (microstructured film) to grow muscle fibers. The microstructured edible films were made using micromolding technology. A micromold was tailor-made using a laser cutting machine to obtain parallel fibers with a diameter in the range of 70-90 µm. Edible films were made by means of solvent casting using non-mammalian biopolymers. Myoblasts were cultured on flat and microstructured films at three cell densities. Cells on the microstructured films grew with a muscle fiber morphology, but in the case of using the flat film, they only produced unorganized cell proliferation. Myogenic markers were assessed using quantitative polymerase chain reaction. After 14 days, the expression of desmin, myogenin, and myosin heavy chain were significantly higher in microstructured films compared to the flat films. The formation of fiber morphology and the high expression of myogenic markers indicated that a microstructured edible film can be used for the production of in vitro meat.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.