ArticleNPJ science of food2019
Muscle tissue engineering in fibrous gelatin: implications for meat analogs.
Article in NPJ science of food, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
64 citing papers in PubMed, 196 citations in OpenAlex.
- A Comprehensive Review on Food-Grade Electrospinning of Natural Biopolymers for Cultivated Meat Applications.Foods (Basel, Switzerland) · 2026Review
- Cultivated Meat and the Future of Food Systems: Promise, Progress, and Challenges.Food science & nutrition · 2026Review
- Advances in porcine stem cell research and their applications in agriculture.Frontiers in cell and developmental biology · 2026Review
- From Muscle Fibers to Functional Foods: Bridging Conventional and Cultivated Approaches.Advances in biochemical engineering/biotechnology · 2026Review
- Edible Scaffolds for Cultivated Meat Production.Advances in biochemical engineering/biotechnology · 2026Review
- Animal-derived components in cultivated meat research and their alternatives.NPJ science of food · 2025Review
- A scaffold based on uncoated rice grains integrated with piscine cells supporting the production of a sustainable food.NPJ science of food · 2025Article
- From nature to plate: harnessing naturally abundant scaffolds for sustainable production of protein-inspired cultivated snack.NPJ science of food · 2025Article
- Curcumin-incorporated edible hydrogel films based on potato starch/κ-carrageenan/poly(vinyl alcohol) for cultured meat scaffolding.Biomedical engineering online · 2025Article
- Engineering Processes for Plant-Based Meat Analogs: Current Status and Future Outlook.Comprehensive reviews in food science and food safety · 2025Review
- Trends in non-animal scaffolds for cultured meat structuration.NPJ science of food · 2025Review
- 3D bioprinting of plant and animal cell-based hybrid food.Nature communications · 2025Article
- Advancements in Stem Cell Applications for Livestock Research: A Review.Veterinary sciences · 2025Review
- Advancing biohybrid robotics: Innovations in contraction models, control techniques, and applications.Biophysics reviews · 2025Review
- Growing meat on autoclaved vegetables with biomimetic stiffness and micro-patterns.Nature communications · 2025Article
- Studying the impact of zein microfibers on the physicochemical and microstructural properties of bi-gels based on ι-carrageenan hydrogels and beeswax oleogels.Current research in food science · 2025Article
- Fundamental study on structural formation, amino acids and nucleotide-related compounds of cultivated meat from 3D-cultured pig muscle stem cells.Food science and biotechnology · 2025Article
- Future protein alternative: recent progress and challenges in cellular agriculture.Food science and biotechnology · 2025Review
- Cutting-Edge Technologies of Meat Analogs: A Review.Food science of animal resources · 2025Review
- Advancements in skeletal muscle tissue engineering: strategies for repair and regeneration of skeletal muscle beyond self-repair.Regenerative biomaterials · 2025Review
4 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 1 institution in 1 country.
Funding
Abstract
Bioprocessing applications that derive meat products from animal cell cultures require food-safe culture substrates that support volumetric expansion and maturation of adherent muscle cells. Here we demonstrate scalable production of microfibrous gelatin that supports cultured adherent muscle cells derived from cow and rabbit. As gelatin is a natural component of meat, resulting from collagen denaturation during processing and cooking, our extruded gelatin microfibers recapitulated structural and biochemical features of natural muscle tissues. Using immersion rotary jet spinning, a dry-jet wet-spinning process, we produced gelatin fibers at high rates (~ 100 g/h, dry weight) and, depending on process conditions, we tuned fiber diameters between ~ 1.3 ± 0.1 μm (mean ± SEM) and 8.7 ± 1.4 μm (mean ± SEM), which are comparable to natural collagen fibers. To inhibit fiber degradation during cell culture, we crosslinked them either chemically or by co-spinning gelatin with a microbial crosslinking enzyme. To produce meat analogs, we cultured bovine aortic smooth muscle cells and rabbit skeletal muscle myoblasts in gelatin fiber scaffolds, then used immunohistochemical staining to verify that both cell types attached to gelatin fibers and proliferated in scaffold volumes. Short-length gelatin fibers promoted cell aggregation, whereas long fibers promoted aligned muscle tissue formation. Histology, scanning electron microscopy, and mechanical testing demonstrated that cultured muscle lacked the mature contractile architecture observed in natural muscle but recapitulated some of the structural and mechanical features measured in meat products.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.