ArticleMolecules (Basel, Switzerland)2019
An Effective Method of Isolating Honey Proteins.
Article in Molecules (Basel, Switzerland), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Strong antimicrobial activity and unique physicochemical characteristics in honey from Australian stingless beesApplied and environmental microbiology · 2025Article
- Honeybee proteins in Saudi honeys: A shotgun gel-free proteomic study.Food chemistry: X · 2025Article
- Development of an LC-MS/MS Method for Quantitation of Western Honeybee (Journal of applied glycoscience · 2025Article
- Antioxidant activities of Saudi honey samples related to their content of short peptides.Scientific reports · 2024Article
- Article
- The Comparison of Honey Enriched with Laboratory Fermented Pollen vs. Natural Bee Bread in Terms of Nutritional and Antioxidant Properties, Protein In Vitro Bioaccessibility, and Its Genoprotective Effect in Yeast Cells.Molecules (Basel, Switzerland) · 2023Article
- Chemical Composition and Bioactivity of Laboratory-Fermented Bee Pollen in Comparison with Natural Bee Bread.Biomolecules · 2023Article
- Integrated Approach to Extract and Purify Proteins from Honey by Ionic Liquid-Based Three-Phase Partitioning.ACS sustainable chemistry & engineering · 2022Article
- Antimicrobial and antioxidant activity of proteins isolated fromFood chemistry: X · 2022Article
- Determination of Floral Origin Markers of Latvian Honey by Using IRMS, UHPLC-HRMS, andFoods (Basel, Switzerland) · 2021Article
- Honey and Its Antimicrobial Properties: A Function of a Single Component, or the Sum of Its Parts?Cureus · 2021Article
- The Comparison of Physicochemical Parameters, Antioxidant Activity and Proteins for the Raw Local Polish Honeys and Imported Honey Blends.Molecules (Basel, Switzerland) · 2021Article
- Hydrolysates from bee pollen could induced apoptosis in human bronchogenic carcinoma cells (ChaGo-K-1).Journal of food science and technology · 2021Article
- Modern Methods for Assessing the Quality of Bee Honey and Botanical Origin Identification.Foods (Basel, Switzerland) · 2020Review
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Authors and funding
5 authors.
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Abstract
Honey is a natural sweetener composed mostly of sugars, but it contains also pollen grains, proteins, free amino acids, and minerals. The amounts and proportions of these components depend on the honey type and bee species. Despite the low content of honey protein, they are becoming a popular study object, and have recently been used as markers of the authenticity and quality of honey. Currently, the most popular methods of protein isolation from honey are dialysis against distilled water, lyophilization of dialysate, or various precipitation protocols. In this work, we propose a new method based on saturated phenol. We tested it on three popular polish honey types and we proved its compatibility with both 1D and 2D polyacrylamide gel electrophoresis (PAGE) and MS (mass spectrometry) techniques. The elaborated technique is also potentially less expensive and less time-consuming than other previously described methods, while being equally effective.
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