ArticleFrontiers in plant science2019
Hordein Accumulation in Developing Barley Grains.
Article in Frontiers in plant science, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 41 citations in OpenAlex.
- Genetic Architecture of Grain Protein Content in Hulless Barley: Insights from Multi-Environment GWAS.Plants (Basel, Switzerland) · 2026Article
- Mining and Validation of Novel Umami Peptides in Non-Alcoholic Beer by Integrating Machine Learning Prediction, Molecular Docking, and Sensory Validation, and Their Multidimensional Sensory Impacts on Beer Body.Foods (Basel, Switzerland) · 2026Article
- Physiological and Molecular Mechanisms of Nitrogen Regulation on Grain Quality in Cereal Crops at Later Stages.International journal of molecular sciences · 2026Review
- Improvement of cereal- and legume-derived protein quality with selenium and sulfur for plant food production.Journal of the science of food and agriculture · 2025Review
- Simplex and multiplex CRISPR/Cas9-mediated knockout of grain protease inhibitors in model and commercial barley improves hydrolysis of barley and soy storage proteins.Plant biotechnology journal · 2025Article
- Immunolocalization of hordein synthesis and transport in developing barley endosperm.Plant direct · 2024Article
- TcSERPIN, an inhibitor that interacts with cocoa defense proteins and has biotechnological potential against human pathogens.Frontiers in plant science · 2024Article
- Lable-free aptamer portable colorimetric smartphone for gliadin detection in food.Frontiers in bioengineering and biotechnology · 2024Article
- Plant Serpins: Potential Inhibitors of Serine and Cysteine Proteases with Multiple Functions.Plants (Basel, Switzerland) · 2023Review
- Proteome Changes Resulting from Malting in Hordein-Reduced Barley Lines.Journal of agricultural and food chemistry · 2023Article
- Extraction, Composition, Functionality, and Utilization of Brewer's Spent Grain Protein in Food Formulations.Foods (Basel, Switzerland) · 2023Review
- Application of Mass Spectrometry-Based Proteomics to Barley Research.Journal of agricultural and food chemistry · 2021Review
- Multi-Omics Strategies for Decoding Smoke-Assisted Germination Pathways and Seed Vigour.International journal of molecular sciences · 2020Review
- Scientific Opinion related to a notification from Lyckeby Starch AB on barley starch to be used in the manufacturing of several foods as ingredient, of the food additive modified starch and of glucose syrups pursuant to Article 21(2) of Regulation (EU) No 1169/2011 - for permanent exemption from labelling.EFSA journal. European Food Safety Authority · 2020Article
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The temporal pattern of accumulation of hordein storage proteins in developing barley grains was studied by enzyme-linked immunosorbent assay (ELISA), western blot and liquid chromatography tandem mass spectrometry (LC-MS/MS). Hordein accumulation was compared to the pattern seen for two abundant control proteins, serpin Z4 (an early accumulator) and lipid transferase protein (LTP1, a late accumulator). Hordeins were detected from 6 days post-anthesis (DPA) and peaked at 30 DPA. Changes in fresh weight indicate that desiccation begins at 20 DPA and by 37 DPA fresh weight had decreased by 35%. ELISA analysis of hordein content, expressed on a protein basis, increased to a maximum at 30 DPA followed by a 17% decrease by 37 DPA. The accumulation of 39 tryptic and 29 chymotryptic hordein peptides representing all classes of hordein was studied by LC-MS/MS. Most peptides increased to a maximum at 30 DPA, and either remained at the maximum or did not decrease significantly. Only five tryptic peptides, members of the related B1- and γ1-hordeins decreased significantly by 21-51% at 37 DPA. Thus, the concentration of some specific peptides was reduced while remaining members of the same family were not affected. The N-terminal signal region was removed by proteolysis during co-translation. In addition to a suite of previously characterized hordeins, two novel barley B-hordein isoforms mapping to wheat low molecular weight glutenins (LMW-GS-like B-hordeins), and two avenin-like proteins (ALPs) sharing homology with wheat ALPs, were identified. These identified isoforms have not previously been mapped in the barley genome. Cereal storage proteins provide significant nutritional content for human consumption and seed germination. In barley, the bulk of the storage proteins comprise the hordein family and the final hordein concentration affects the quality of baked and brewed products. It is therefore important to study the accumulation of hordeins as this knowledge may assist plant breeding for improved health outcomes (by minimizing triggering of detrimental immune responses), nutrition and food processing properties.
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