ArticlePlant biotechnology journal2019
Cas9-mediated mutagenesis of potato starch-branching enzymes generates a range of tuber starch phenotypes.
Article in Plant biotechnology journal, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
64 citing papers in PubMed, 152 citations in OpenAlex.
- Integrated transcriptomic and metabolomic analysis reveals candidate regulatory networks associated with starch accumulation in tetraploid potato.Functional & integrative genomics · 2026Article
- Overcoming breeding barriers with genome editing in autopolyploid crops.BMC plant biology · 2026Review
- Advances in CRISPR Plant Applications.International journal of molecular sciences · 2026Review
- Starch Synthase 3 isoforms are essential for normal starch granule initiation in wheat endosperm.The New phytologist · 2026Article
- GeARF5/GeIAA33-GeSWEET14 module balances the secondary metabolic biosynthesis to increase the yield and quality in Gastrodia elata.Journal of integrative plant biology · 2026Article
- Modification of starch synthesis in food crops using CRISPR/Cas9 gene editing technology for changing climate.Stress biology · 2026Review
- Commentary: CRISPR-Cas9 mediated editing of starch branching enzyme, SBE2 gene in potato for enhanced resistant starch for health benefits.Frontiers in genome editing · 2026Article
- The miR395b-ABI5 module regulates amylopectin branching and biosynthesis and affects lotus root quality.Plant physiology · 2025Article
- Biofortification of potato nutrition.Journal of advanced research · 2025Review
- Comprehensive analysis of RNA-seq data reveals novel insight in the formation of bamboo embryogenic callus in Bambusa changningensis.BMC plant biology · 2025Article
- CRISPR-Cas Gene Editing Technology in Potato.International journal of molecular sciences · 2025Review
- Integrating genome editing with omics, artificial intelligence, and advanced farming technologies to increase crop productivity.Plant communications · 2025Review
- Food as Medicine: Curbing Type-2 Diabetes Prevalence Through Consumption of High Amylose Starchy Foods in Sub-Saharan Africa.Advanced pharmaceutical bulletin · 2025Review
- Simultaneous Repression of GLUCAN WATER DIKINASE 1 and STARCH BRANCHING ENZYME 1 in Potato Tubers Leads to Starch With Increased Amylose and Novel Industrial Properties.Biotechnology journal · 2025Article
- Physiological and compensatory roles of three starch-branching enzymes in different rice organs.Plant molecular biology · 2025Article
- Distinct effects of PTST2b and MRC on starch granule morphogenesis in potato tubers.Plant biotechnology journal · 2025Article
- Advancing vegetable genetics with gene editing: a pathway to food security and nutritional resilience in climate-shifted environments.Functional & integrative genomics · 2025Review
- The transcription factor NnNAC100 positively regulates amylopectin biosynthesis by activating NnSBEII in the rhizome of Nelumbo nucifera Gaertn.Plant cell reports · 2025Article
- CRISPR-Cas9 mediated editing ofFrontiers in genome editing · 2025Article
- The Potential of CRISPR/Cas9 to Circumvent the Risk Factor NeurotoxinCurrent issues in molecular biology · 2024Review
4 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
We investigated whether Cas9-mediated mutagenesis of starch-branching enzymes (SBEs) in tetraploid potatoes could generate tuber starches with a range of distinct properties. Constructs containing the Cas9 gene and sgRNAs targeting SBE1, SBE2 or both genes were introduced by Agrobacterium-mediated transformation or by PEG-mediated delivery into protoplasts. Outcomes included lines with mutations in all or only some of the homoeoalleles of SBE genes and lines in which homoeoalleles carried several different mutations. DNA delivery into protoplasts resulted in mutants with no detectable Cas9 gene, suggesting the absence of foreign DNA. Selected mutants with starch granule abnormalities had reductions in tuber SBE1 and/or SBE2 protein that were broadly in line with expectations from genotype analysis. Strong reduction in both SBE isoforms created an extreme starch phenotype, as reported previously for low-SBE potato tubers. HPLC-SEC and
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.