Evidence map›Paper›PMID 30787608›Full record

ArticleInternational journal of nanomedicine2019

Enhanced growth and differentiation of myoblast cells grown on E-jet 3D printed platforms.

Haoxiang Chen, Juchang Zhong, Jian Wang, Ruiying Huang, Xiaoyin Qiao, Honghui Wang, Zhikai Tan

Abstract read
In one paragraph

Article in International journal of nanomedicine, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed.

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  13. Scaffolding Biomaterials for 3D Cultivated Meat: Prospects and Challenges.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022
    Review
  14. Old and new biomarkers for volumetric muscle loss.Current opinion in pharmacology · 2021
    Review
  15. Review
  16. Next Stage Approach to Tissue Engineering Skeletal Muscle.Bioengineering (Basel, Switzerland) · 2020
    Review
  17. Review
  18. Article
  19. BioengineeredJournal of tissue engineering
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Haoxiang ChenCollege of Biology, Hunan University, Changsha, Hunan 410082, China, tanzk@hnu.edu.cn.
Juchang ZhongCollege of Biology, Hunan University, Changsha, Hunan 410082, China, tanzk@hnu.edu.cn.
Jian WangCollege of Biology, Hunan University, Changsha, Hunan 410082, China, tanzk@hnu.edu.cn.
Ruiying HuangCollege of Biology, Hunan University, Changsha, Hunan 410082, China, tanzk@hnu.edu.cn.
Xiaoyin QiaoCollege of Biology, Hunan University, Changsha, Hunan 410082, China, tanzk@hnu.edu.cn.
Honghui WangCollege of Biology, Hunan University, Changsha, Hunan 410082, China, tanzk@hnu.edu.cn.
Zhikai TanCollege of Biology, Hunan University, Changsha, Hunan 410082, China, tanzk@hnu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundSkeletal muscle tissue engineering often involves the prefabrication of muscle tissues in vitro by differentiation and maturation of muscle precursor cells on a platform which provides an environment that facilitates the myogenic differentiation of the seeded cells.

methodsPoly lactic-co-glycolic acid (PLGA) 3D printed scaffolds, which simulate the highly complex structure of extracellular matrix (ECM), were fabricated by E-jet 3D printing in this study. The scaffolds were used as platforms, providing environment that aids in growth, differentiation and other properties of C2C12 myoblast cells.

resultsThe C2C12 myoblast cells grown on the PLGA 3D printed platforms had enhanced cell adhesion and proliferation. Moreover, the platforms were able to induce myogenic differentiation of the myoblast cells by promoting the formation of myotubes and up-regulating the expressions of myogenic genes (MyHC and MyOG).

conclusionThe fabricated 3D printed platforms have excellent biocompatibility, thereby can potentially be used as functional cell culture platforms in skeletal tissue engineering and regeneration.

Indexed as

Cell DifferentiationHydrodynamicsPrinting, Three-DimensionalAnimalsCell AdhesionCell Culture TechniquesCell ProliferationMuscle, SkeletalMyoblastsPolylactic Acid-Polyglycolic Acid CopolymerTissue EngineeringTissue ScaffoldsPolylactic Acid-Polyglycolic Acid Copolymer3D cell culture3D printingmyoblastmyogenic differentiationskeletal muscle regeneration

Identifiers

PMID30787608
PMCPMC6366362

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.