ArticleMethodsX2018
Improved method for qualitative screening of lipolytic bacterial strains.
Article in MethodsX, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 49 citations in OpenAlex.
- Heterologous expression of lipase gene in Cereibacter sphaeroides 2.4.1 driven by an anaerobic Photosynthesis-Inducible promoter.Journal, genetic engineering & biotechnology · 2026Article
- Whole-genome characterization and analysis of Pantoea agglomerans R6: a genomic insight into its pathogenicity and resistance as a potential opportunistic plant pathogen.BMC genomics · 2026Article
- Genome mining and screening for plastic-degrading potential in marine bacteria.Applied microbiology and biotechnology · 2026Article
- Comparative genomics-based insights intoApplied and environmental microbiology · 2025Article
- Shifting the pH profiles of Staphylococcus epidermidis lipase (SEL) and Staphylococcus hyicus lipase (SHL) through generating chimeric lipases by DNA shuffling strategy.World journal of microbiology & biotechnology · 2024Article
- Evaluation of the Differential Postbiotic Potential of Shewanella putrefaciens Pdp11 Cultured in Several Growing Conditions.Marine biotechnology (New York, N.Y.) · 2024Article
- A Combination of Blue Light at 460 nm and HMicroorganisms · 2023Article
- Rhizopus stolonifer biomass catalytic transesterification capability: optimization of cultivation conditions.Microbial cell factories · 2023Article
- Microbial Ecology of Sheep Milk, Artisanal Feta, and Kefalograviera Cheeses. Part II: Technological, Safety, and Probiotic Attributes of Lactic Acid Bacteria Isolates.Foods (Basel, Switzerland) · 2022Article
- Technological and Safety Attributes of Lactic Acid Bacteria and Yeasts Isolated from Spontaneously Fermented Greek Wheat Sourdoughs.Microorganisms · 2021Article
- Multi-Functional Potential of Presumptive Lactic Acid Bacteria Isolated from Chihuahua Cheese.Foods (Basel, Switzerland) · 2020Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Esterases and lipases are lipolytic enzymes that catalyse the hydrolysis of triacylglycerols, Determination of lipolysis on agar plates is a simple approach to determine lipase or esterase action, but visual evaluation of lipolysis is frequently difficult in practice. Therefore, the aim of this work was to improve the efficiency of lipolysis visualization in tributyrin agar (mTBA) by adding calcium and/or magnesium ions in the screening of lipolytic microbial strains. Lipolytic activity was evaluated in mTBA using the well diffusion technique, where a clear zone around the inoculated wells indicated lipid hydrolysis. Results suggest that the addition of 2.5 mM calcium and 5.0 mM magnesium was the best combination of ion addition to TBA. Lipolytic activity increased the clearing zone up to 38% more than without the addition of ions and the clear zone was clearly observed. The mTBA plate was used with culture collection microbial strains, as well as with a collection of soil microorganisms, to identify lipase producers. The addition of calcium and magnesium ions can provide an easier screening procedure for selection of lipolytic bacterial strains. •A modified tributyrin agar for screening of lipolytic bacteria was prepared by adding calcium and magnesium ions.•The modified TBA agar was tested with control bacterial strains, and, based on the results, 2.5 mM Ca and 5.0 mM Mg ions were added in the mTBA.•mTBA was validated with environmental bacterial strains for screening of lipolytic activity.
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