ArticlePLoS neglected tropical diseases2018
Detection of Bartonella spp. in fleas by MALDI-TOF MS.
Article in PLoS neglected tropical diseases, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it, 46 citations in OpenAlex.
- MALDI-TOF mass spectrometry as a diagnostic tool in human and veterinary helminthology: a systematic review.Parasites & vectors · 2019Pooled it
- Hidden Burden ofOpen forum infectious diseases · 2024Review
- Omics for deciphering oral microecology.International journal of oral science · 2024Review
- Using MALDI-TOF MS to Identify Mosquitoes from Senegal and the Origin of Their Blood Meals.Insects · 2023Article
- Identification of Vietnamese Flea Species and Their Associated Microorganisms Using Morphological, Molecular, and Protein Profiling.Microorganisms · 2023Article
- Review
- Applications of MALDI-TOF Mass Spectrometry to the Identification of Parasites and Arthropod Vectors of Human Diseases.Microorganisms · 2022Review
- Enhanced procedures for mosquito identification by MALDI-TOF MS.Parasites & vectors · 2022Article
- Use of the proteomic tool MALDI-TOF MS in termite identification.Scientific reports · 2022Article
- Predicting global potential distribution ofFrontiers in public health · 2022Article
- Wolbachia detection in Aedes aegypti using MALDI-TOF MS coupled to artificial intelligence.Scientific reports · 2021Article
- Bartonella gabonensis sp. nov., a new bartonella species from savannah rodent Lophuromys sp. in Franceville, Gabon.New microbes and new infections · 2020Article
- Development of MALDI-TOF mass spectrometry for the identification of lice isolated from farm animals.Parasite (Paris, France) · 2020Article
- Identification of French Guiana sand flies using MALDI-TOF mass spectrometry with a new mass spectra library.PLoS neglected tropical diseases · 2019Article
- Effectiveness of a 10% imidacloprid/4.5% flumethrin polymer matrix collar in reducing the risk of Bartonella spp. infection in privately owned cats.Parasites & vectors · 2019Article
- Use of MALDI-TOF MS for the Identification of Chad Mosquitoes and the Origin of Their Blood Meal.The American journal of tropical medicine and hygiene · 2019Article
- Shortening Fermentation Period and Quality Improvement of Fermented Fish,Frontiers in microbiology · 2018Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMatrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) has recently emerged in the field of entomology as a promising method for the identification of arthropods and the detection of associated pathogens. METHODOLOGY/PRINCIPAL
findingsAn experimental model of Ctenocephalides felis (cat fleas) infected with Bartonella quintana and Bartonella henselae was developed to evaluate the efficacy of MALDI-TOF MS in distinguishing infected from uninfected fleas, and its ability to distinguish fleas infected with Bartonella quintana from fleas infected with Bartonella henselae. For B. quintana, two groups of fleas received three successive blood meals, infected or not. A total of 140 fleas (100 exposed fleas and 40 control fleas) were engorged on human blood, infected or uninfected with B. quintana. Regarding the second pathogen, two groups of fleas (200 exposed fleas and 40 control fleas) were fed in the same manner with human blood, infected or not with Bartonella henselae. Fleas were dissected longitudinally; one-half was used for assessment of B. quintana and B. henselae infectious status by real-time PCR, and the second half was subjected to MALDI-TOF MS analysis. Comparison of MS spectra from infected fleas and uninfected fleas revealed distinct MS profiles. Blind queries against our MALDI-TOF MS arthropod database, upgraded with reference spectra from B. quintana and B. henselae infected fleas but also non-infected fleas, provided the correct classification for 100% of the different categories of specimens tested on the first model of flea infection with Bartonella quintana. As for Bartonella henselae, 81% of exposed qPCR-positive fleas, 96% of exposed qPCR-negative fleas and 100% of control fleas were correctly identified on the second model of flea infection. MALDI-TOF MS successfully differentiated Bartonella spp.-infected and uninfected fleas and was also able to correctly differentiate fleas infected with Bartonella quintana and fleas infected with Bartonella henselae. MALDI-TOF MS correctly identified flea species as well as their infectious status, consistent with the results of real-time PCR. CONCLUSIONS/SIGNIFICANCE: MALDI-TOF is a promising tool for identification of the infection status of fleas infected with Bartonella spp., which allows new possibilities for fast and accurate diagnosis in medical entomology and vector surveillance.
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