ArticleProbiotics and antimicrobial proteins2019
Strain Screening from Traditional Fermented Soybean Foods and Induction of Nattokinase Production in Bacillus subtilis MX-6.
Article in Probiotics and antimicrobial proteins, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 30 citations in OpenAlex.
- Valorization of tofu-processing wastewater for sustainable nattokinase production: insights from integrated multi-omics analysis.World journal of microbiology & biotechnology · 2026Article
- Enhanced nattokinase production by optimized fermentation conditions of douchi-derived Bacillus subtilis natto NK11 and evaluation of its thrombolytic activity.Antonie van Leeuwenhoek · 2025Article
- Research Progress of Nattokinase in Reducing Blood Lipid.Nutrients · 2025Review
- Article
- Modeling andHeliyon · 2023Article
- Effect of fermentation on structural properties and antioxidant activity of wheat gluten byFrontiers in nutrition · 2023Article
- High and Economical Nattokinase Production with Acetoin as a Useful Byproduct from Soybean Milk and Glucose.Probiotics and antimicrobial proteins · 2022Article
- Characterization of a Nattokinase from the Newly Isolated Bile Salt-ResistantFoods (Basel, Switzerland) · 2022Article
- Biotechnology, Bioengineering and Applications ofBiomolecules · 2022Review
- Recent Advances in Nattokinase-Enriched Fermented Soybean Foods: A Review.Foods (Basel, Switzerland) · 2022Review
- Tofu processing wastewater as a low-cost substrate for high activity nattokinase production using Bacillus subtilis.BMC biotechnology · 2021Article
- Microencapsulation of nattokinase from fermentation by spray drying: Optimization, comprehensive score, and stability.Food science & nutrition · 2021Article
- Increase of a Fibrinolytic Enzyme Production through Promoter Replacement ofJournal of microbiology and biotechnology · 2021Article
- Study on the mechanism of production of γ-PGA and nattokinase in Bacillus subtilis natto based on RNA-seq analysis.Microbial cell factories · 2021Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The plasminogen-free fibrin plate assay method was used to isolate Bacillus subtilis MX-6, a strain with high production of nattokinase from Chinese douchi. The presence of aprN, a gene-encoding nattokinase, was verified with PCR method. The predicted amino acid sequence was aligned with homologous sequences, and a phylogenetic tree was constructed. Nattokinase was sublimated with ammonium sulfate, using a DEAE-Sepharose Fast Flow column, a CM-Sepharose Fast Flow column and a Sephadex G-75 gel filtration column. SDS-PAGE analysis indicated that the molecular weight of the purified nattokinase from Bacillus subtilis MX-6 was about 28 kDa. Fermentation of Bacillus subtilis MX-6 nattokinase showed that nattokinase production was maximized after 72 h; the diameter of clear zone reached 21.60 mm on the plasminogen-free fibrin plate. Nattokinase production by Bacillus subtilis MX-6 increased significantly after supplementation with supernatant I, supernatant II and soy peptone but decreased substantially after the addition of amino acids. This result indicated that the nattokinase production by B. subtilis MX-6 might be induced by soybean polypeptides. The addition of MgSO
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