ArticleThe New phytologist2018
Vacuolar processing enzyme 4 contributes to maternal control of grain size in barley by executing programmed cell death in the pericarp.
Article in The New phytologist, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 33 citations in OpenAlex.
- 5-azacytidine affects triticale microspore embryogenesis and is associated with modulation of reactive oxygen species, cysteine protease profiles, and programmed cell death.BMC plant biology · 2026Article
- Plant caspase-like proteins: from function identification to application in winter rapeseed genetic breeding.Frontiers in plant science · 2026Review
- Enhanced Oligopeptide and Free Tryptophan Release from Chickpea and Lentil Proteins: A Comparative Study of Enzymatic Modification with Bromelain, Ficin, and Papain.Plants (Basel, Switzerland) · 2024Article
- A NAC-EXPANSIN module enhances maize kernel size by controlling nucellus elimination.Nature communications · 2022Article
- Quantitative monitoring of paramagnetic contrast agents and their allocation in plant tissues via DCE-MRI.Plant methods · 2022Article
- Integrative analysis of transcriptome and miRNAome reveals molecular mechanisms regulating pericarp thickness in sweet corn during kernel development.Frontiers in plant science · 2022Article
- Programmed Cell Death in DevelopingInternational journal of molecular sciences · 2021Article
- Article
- Grain filling in barley relies on developmentally controlled programmed cell death.Communications biology · 2021Article
- Programmed Degradation of Pericarp Cells in Wheat Grains Depends on Autophagy.Frontiers in genetics · 2021Article
- Protein sorting into protein bodies during barley endosperm development is putatively regulated by cytoskeleton members, MVBs and the HvSNF7s.Scientific reports · 2020Article
- Comparison ofAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Article
- Genome-Wide Identification and Characterization of Vacuolar Processing Enzyme Gene Family and Diverse Expression Under Stress in Apple (Frontiers in plant science · 2020Article
- Activity profiling of barley vacuolar processing enzymes provides new insights into the plant and cyst nematode interaction.Molecular plant pathology · 2020Article
- A reductionist approach to dissecting grain weight and yield in wheat.Journal of integrative plant biology · 2019Review
- Plant Proteases: From Key Enzymes in Germination to Allies for Fighting Human Gluten-Related Disorders.Frontiers in plant science · 2019Article
- Plant Vacuolar Processing Enzymes.Frontiers in plant science · 2019Review
- Seed tissue and nutrient partitioning, a case for the nucellus.Plant reproduction · 2018Review
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Authors and funding
14 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The angiosperm embryo and endosperm are limited in space because they grow inside maternal seed tissues. The elimination of cell layers of the maternal seed coat by programmed cell death (PCD) could provide space and nutrition to the filial organs. Using the barley (Hordeum vulgare L.) seed as a model, we elucidated the role of vacuolar processing enzyme 4 (VPE4) in cereals by using an RNAi approach and targeting the enzymatic properties of the recombinant protein. A comparative characterization of transgenic versus wild-type plants included transcriptional and metabolic profiling, flow cytometry, histology and nuclear magnetic imaging of grains. The recombinant VPE4 protein exhibited legumain and caspase-1 properties in vitro. Pericarp disintegration was delayed in the transgenic grains. Although the VPE4 gene and enzymatic activity was decreased in the early developing pericarp, storage capacity and the size of the endosperm and embryo were reduced in the mature VPE4-repressed grains. The persistence of the pericarp in the VPE4-affected grains constrains endosperm and embryo growth and leads to transcriptional reprogramming, perturbations in signalling and adjustments in metabolism. We conclude that VPE4 expression executes PCD in the pericarp, which is required for later endosperm filling, and argue for a role of PCD in maternal control of seed size in cereals.
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