ArticleMolecules (Basel, Switzerland)2017
Improved Method for Reliable HMW-GS Identification by RP-HPLC and SDS-PAGE in Common Wheat Cultivars.
Article in Molecules (Basel, Switzerland), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Development of PCR-based markers for the identification of wheat HMW glutenin subunit alleles at the GLU-A1 and GLU-D1 loci.Scientific reports · 2025Article
- High variation in the gluten composition and grain protein content among synthetic wheat lines.PloS one · 2025Article
- Development of PCR-based markers for identification of wheat HMW glutenin Glu-1Bx and Glu-1By alleles.BMC plant biology · 2024Article
- Synergizing breeding strategies via combining speed breeding, phenotypic selection, and marker-assisted backcrossing for the introgression ofFrontiers in plant science · 2024Article
- Generation of Wheat Near-Isogenic Lines Overexpressing 1Bx7 Glutenin with Increased Protein Contents and SDS-Sedimentation Values.Plants (Basel, Switzerland) · 2023Article
- Promoter DNA hypermethylation ofJournal of advanced research · 2022Article
- A Rapid, Reliable RP-UPLC Method for Large-Scale Analysis of Wheat HMW-GS Alleles.Molecules (Basel, Switzerland) · 2021Article
- Proteomic Determination of Low-Molecular-Weight Glutenin Subunit Composition in Aroona Near-Isogenic Lines and Standard Wheat Cultivars.International journal of molecular sciences · 2021Article
- High-throughput analysis of high-molecular weight glutenin subunits in 665 wheat genotypes using an optimized MALDI-TOF-MS method.3 Biotech · 2021Article
- Rapid and Easy High-Molecular-Weight Glutenin Subunit Identification System by Lab-on-a-Chip in Wheat (Plants (Basel, Switzerland) · 2020Article
- Development of an Optimized MALDI-TOF-MS Method for High-Throughput Identification of High-Molecular-Weight Glutenin Subunits in Wheat.Molecules (Basel, Switzerland) · 2020Article
- A New Approach of Extraction of α-Amylase/trypsin Inhibitors from Wheat (Molecules (Basel, Switzerland) · 2019Article
- Genetic Variation for Protein Content and Yield-Related Traits in a Durum Population Derived From an Inter-Specific Cross Between Hexaploid and Tetraploid Wheat Cultivars.Frontiers in plant science · 2019Article
- Isolation and Evaluation of Bioactive Protein and Peptide from Domestic Animals' Bone Marrow.Molecules (Basel, Switzerland) · 2018Article
- Cellular Localization of Wheat High Molecular Weight Glutenin Subunits in Transgenic Rice Grain.International journal of molecular sciences · 2017Article
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Authors and funding
6 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The accurate identification of alleles for high-molecular weight glutenins (HMW-GS) is critical for wheat breeding programs targeting end-use quality. RP-HPLC methods were optimized for separation of HMW-GS, resulting in enhanced resolution of 1By and 1Dx subunits. Statistically significant differences in retention times (RTs) for subunits corresponding to HMW-GS alleles were determined using 16 standard wheat cultivars with known HMW-GS compositions. Subunits that were not identified unambiguously by RP-HPLC were distinguished by SDS-PAGE or inferred from association with linked subunits. The method was used to verify the allelic compositions of 32 Korean wheat cultivars previously determined using SDS-PAGE and to assess the compositions of six new Korean cultivars. Three cultivars contained subunits that were identified incorrectly in the earlier analysis. The improved RP-HPLC method combined with conventional SDS-PAGE provides for accurate, efficient and reliable identification of HMW-GS and will contribute to efforts to improve wheat end-use quality.
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