ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2017
Antimicrobial effects of Manuka honey on in vitro biofilm formation by Clostridium difficile.
Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 46 citations in OpenAlex.
- A longitudinal analysis of Clostridioides difficile virulence profiles across three geographic regions in Israel (2020-2022).Annals of clinical microbiology and antimicrobials · 2026Article
- Biofilm- and Spore-Disruptive Star-Shaped Poly(l-lysine)/Hyaluronic Acid Microgels for Targeted Oral Therapy ofBiomacromolecules · 2026Article
- Review
- Antimicrobial Effects of Some Natural Products on Adhesion and Biofilm Inhibition ofPharmaceutics · 2024Article
- The Effects of Encapsulation on the In Vitro Anti-Clostridial Activity of Olive Mill Wastewater Polyphenolic Extracts: A Promising Strategy to Limit Microbial Growth in Food Systems.Molecules (Basel, Switzerland) · 2024Article
- The Effect of Subinhibitory Concentration of Metronidazole on the Growth and Biofilm Formation on ToxigenicPathogens (Basel, Switzerland) · 2023Article
- Beyond the Risk of Biofilms: An Up-and-Coming Battleground of Bacterial Life and Potential Antibiofilm Agents.Life (Basel, Switzerland) · 2023Review
- Combinatorial efficacy of Manuka honey and antibiotics in theFrontiers in cellular and infection microbiology · 2023Article
- The Effect of Selected Bee Products on Adhesion and Biofilm ofMolecules (Basel, Switzerland) · 2022Article
- Physicochemical Characterization and Biological Properties of Pine Honey Produced across Greece.Foods (Basel, Switzerland) · 2022Article
- Effects ofMicroorganisms · 2021Article
- Alimentary and Pharmaceutical Approach to Natural Antimicrobials againstFoods (Basel, Switzerland) · 2021Review
- Mucin-Degrading Microbes Release Monosaccharides That ChemoattractACS infectious diseases · 2021Article
- Antibiofilm Activity of Heather and Manuka Honeys and Antivirulence Potential of Some of Their Constituents on the DsbA1 Enzyme ofAntibiotics (Basel, Switzerland) · 2020Article
- The Bactericidal Activity and Spore Inhibition Effect of Manuka Honey againstAntibiotics (Basel, Switzerland) · 2020Article
- The effect of berberine chloride and/or its combination with vancomycin on the growth, biofilm formation, and motility of Clostridioides difficile.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2020Article
- Evaluation and comparison of the effect of honey, milk and combination of honey-milk on experimental induced second-degree burns of Rabit.Journal of family medicine and primary care · 2020Article
- Article
- Institutional protocols for the oral administration (gavage) of chemicals and microscopic microbial communities to mice: Analytical consensus.Experimental biology and medicine (Maywood, N.J.) · 2019Review
- Antibacterial activity of varying UMF-graded Manuka honeys.PloS one · 2019Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Clostridium difficile is the cause of the nosocomial C. difficile infection (CDI). The conventional antibiotics used in CDI therapy are often unsuccessful, and recurrent infections may occur. Biofilm formation by C. difficile is associated with chronic or recurrent infections; biofilms may contribute to virulence and impaired antimicrobial efficacy. Manuka honey, derived from the Manuka tree (Leptospermum scoparium), is known to exhibit antimicrobial properties that are associated with its significant content of methylglyoxal, a natural antibiotic. The aim of the present study was to determine the antimicrobial effect of Manuka honey on clinical C. difficile strains belonging to four prominent polymerase chain reaction (PCR) ribotypes (RTs) (RT017, RT023, RT027 and RT046) and on their biofilm formation in vitro. Minimal inhibitory and bactericidal concentrations (MICs and MBCs, respectively) were determined using the broth dilution method. The biomass of the biofilm and the clearance of C. difficile biofilms by Manuka honey were determined using the crystal violet staining method. The MIC and MBC of Manuka honey for C. difficile strains were equal at 6.25% (v/v). PCR RT027 strains produced more biofilm in vitro than the other examined strains. Manuka honey effectively inhibited biofilm formation by C. difficile strains of different PCR RTs.
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