ReviewWorld journal of microbiology & biotechnology2017
Microbial production of nattokinase: current progress, challenge and prospect.
Review in World journal of microbiology & biotechnology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 58 citations in OpenAlex.
- System reconstruction ofSynthetic and systems biotechnology · 2027Article
- Liquid fermentation technology optimization of nattokinase: from Lab-scale to Pilot-scale.Bioprocess and biosystems engineering · 2026Article
- Hyperproduction of nattokinase from Bacillus subtilis VIT MS2 using random mutagenesis and statistical optimization through central composite design.BMC microbiology · 2025Article
- Production of a Recombinant Fibrinolytic Protease from an Isolate of Serratia marcescens from the Amazon Basin.ACS omega · 2025Article
- Enhanced thermostability of nattokinase by rational design of disulfide bond.Microbial cell factories · 2025Article
- Enhanced nattokinase production byFrontiers in microbiology · 2025Article
- Review
- Fibrin and Fibrinolytic Enzyme Cascade in Thrombosis: Unravelling the Role.Life (Basel, Switzerland) · 2023Review
- High-Efficiency Fermentation of Nattokinase by RecombinantFoods (Basel, Switzerland) · 2023Article
- Development of a Glycerol-Inducible Expression System for High-Yield Heterologous Protein Production in Bacillus subtilis.Microbiology spectrum · 2022Article
- High and Economical Nattokinase Production with Acetoin as a Useful Byproduct from Soybean Milk and Glucose.Probiotics and antimicrobial proteins · 2022Article
- The potential role of ischaemia-reperfusion injury in chronic, relapsing diseases such as rheumatoid arthritis, Long COVID, and ME/CFS: evidence, mechanisms, and therapeutic implications.The Biochemical journal · 2022Review
- Characterization of a Nattokinase from the Newly Isolated Bile Salt-ResistantFoods (Basel, Switzerland) · 2022Article
- Recent Advances in Nattokinase-Enriched Fermented Soybean Foods: A Review.Foods (Basel, Switzerland) · 2022Review
- Antiprotozoal activity of different Xenorhabdus and Photorhabdus bacterial secondary metabolites and identification of bioactive compounds using the easyPACId approach.Scientific reports · 2022Article
- Tofu processing wastewater as a low-cost substrate for high activity nattokinase production using Bacillus subtilis.BMC biotechnology · 2021Article
- Microencapsulation of nattokinase from fermentation by spray drying: Optimization, comprehensive score, and stability.Food science & nutrition · 2021Article
- Increase of a Fibrinolytic Enzyme Production through Promoter Replacement ofJournal of microbiology and biotechnology · 2021Article
- Purification and Characterization of a Thrombolytic Enzyme Produced by a New Strain ofJournal of microbiology and biotechnology · 2021Article
- Strain Screening from Traditional Fermented Soybean Foods and Induction of Nattokinase Production in Bacillus subtilis MX-6.Probiotics and antimicrobial proteins · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nattokinase (EC 3.4.21.62) is a profibrinolytic serine protease with a potent fibrin-degrading activity, and it has been produced by many host strains. Compared to other fibrinolytic enzymes (urokinase, t-PA and streprokinase), nattokinase shows the advantages of having no side effects, low cost and long life-time, and it has the potential to be used as a drug for treating cardiovascular disease and served as a functional food additive. In this review, we focused on screening of producing strains, genetic engineering, fermentation process optimization for microbial nattokinase production, and the extraction and purification of nattokinase were also discussed in this particular chapter. The selection of optimal nattokinase producing strain was the crucial starting element for improvement of nattokinase production. Genetic engineering, protein engineering, fermentation optimization and process control have been proved to be the effective strategies for enhancement of nattokinase production. Also, extraction and purification of nattokinase are critical for the quality evaluation of nattokinase. Finally, the prospect of microbial nattokinase production was also discussed regarding the recent progress, challenge, and trends in this field.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.