ArticleThe Journal of biological chemistry2017
Galactomannan Catabolism Conferred by a Polysaccharide Utilization Locus of Bacteroides ovatus: ENZYME SYNERGY AND CRYSTAL STRUCTURE OF A β-MANNANASE.
Article in The Journal of biological chemistry, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed, 114 citations in OpenAlex.
- Immunomodulatory Function of Soluble Dietary Fiber: Toward Application for Prevention and Treatment of Food Allergies.Current allergy and asthma reports · 2026Review
- Article
- Metabolism of β-mannans by representative, understudied Bacillota species from the human colon.FEMS microbiology ecology · 2026Article
- Deacetylation of β-Mannans by Two Complementary Carbohydrate Esterases from the Human Gut MicrobeBiochemistry · 2026Article
- Unusual molecular architecture of a human gut microbiota β-mannanase reveals a new CBM family.Cellular and molecular life sciences : CMLS · 2026Article
- Late Stage Mannan Metabolism in Cellvibrio japonicus Requires the Combined Action of a Mannosyl-Glucose Phosphorylase and a Mannobiose Epimerase.Molecular microbiology · 2026Article
- A twin xanthan lyase-dependent xanthan degradation system in Paenibacillus taichungensis I5.Applied microbiology and biotechnology · 2026Article
- Mannobiose Production Through Synergistic Enzymatic Hydrolysis of Locust Bean Gum by Mannanases from Alkaliphilic Bacillus sp. N16-5.Applied biochemistry and biotechnology · 2025Article
- Selective utilization of medicinal polysaccharides by human gut Bacteroides and Parabacteroides species.Nature communications · 2025Article
- Genetic analysis of a β-1,3/1,4-glucan utilization gene cluster inBioscience of microbiota, food and health · 2025Article
- Metagenome-derived SusD-homologs affiliated with Bacteroidota bind to synthetic polymers.Applied and environmental microbiology · 2024Article
- Potential of guar gum as a leaky gut model in broilers: Digestibility, performance, and microbiota responses.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2024Article
- Glucomannan promotesiMeta · 2024Article
- The catabolic specialization of the marine bacteriumApplied and environmental microbiology · 2024Article
- Inducible CRISPR-targeted "knockdown" of human gutProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Towards an understanding of the enzymatic degradation of complex plant mannan structures.World journal of microbiology & biotechnology · 2023Review
- Bacteroides ovatus accelerates metformin-induced vitamin B12 deficiency in type 2 diabetes patients by accumulating cobalamin.NPJ biofilms and microbiomes · 2023Article
- Fungal β-glucan-facilitated cross-feeding activities between Bacteroides and Bifidobacterium species.Communications biology · 2023Article
- Potential of Wood Hemicelluloses and Their Derivates as Food Ingredients.Journal of agricultural and food chemistry · 2023Review
- Identifying glycan consumers in human gut microbiota samples using metabolic labeling coupled with fluorescence-activated cell sorting.Nature communications · 2023Article
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Authors and funding
13 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A recently identified polysaccharide utilization locus (PUL) from Bacteroides ovatus ATCC 8483 is transcriptionally up-regulated during growth on galacto- and glucomannans. It encodes two glycoside hydrolase family 26 (GH26) β-mannanases, BoMan26A and BoMan26B, and a GH36 α-galactosidase, BoGal36A. The PUL also includes two glycan-binding proteins, confirmed by β-mannan affinity electrophoresis. When this PUL was deleted, B. ovatus was no longer able to grow on locust bean galactomannan. BoMan26A primarily formed mannobiose from mannan polysaccharides. BoMan26B had higher activity on galactomannan with a high degree of galactosyl substitution and was shown to be endo-acting generating a more diverse mixture of oligosaccharides, including mannobiose. Of the two β-mannanases, only BoMan26B hydrolyzed galactoglucomannan. A crystal structure of BoMan26A revealed a similar structure to the exo-mannobiohydrolase CjMan26C from Cellvibrio japonicus, with a conserved glycone region (-1 and -2 subsites), including a conserved loop closing the active site beyond subsite -2. Analysis of cellular location by immunolabeling and fluorescence microscopy suggests that BoMan26B is surface-exposed and associated with the outer membrane, although BoMan26A and BoGal36A are likely periplasmic. In light of the cellular location and the biochemical properties of the two characterized β-mannanases, we propose a scheme of sequential action by the glycoside hydrolases encoded by the β-mannan PUL and involved in the β-mannan utilization pathway in B. ovatus. The outer membrane-associated BoMan26B initially acts on the polysaccharide galactomannan, producing comparably large oligosaccharide fragments. Galactomanno-oligosaccharides are further processed in the periplasm, degalactosylated by BoGal36A, and subsequently hydrolyzed into mainly mannobiose by the β-mannanase BoMan26A.
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