ArticleApplied and environmental microbiology2015
Validation of Reference Genes for Transcriptional Analyses in Pleurotus ostreatus by Using Reverse Transcription-Quantitative PCR.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 38 citations in OpenAlex.
- Screening and Validation of Reference Genes for Normalization of qRT-PCR in Rice BLB PathogenGenes · 2025Article
- Transcriptome Analysis Revealed That Cell Wall Regulatory Pathways Are Involved in the Tolerance ofJournal of fungi (Basel, Switzerland) · 2025Article
- Identification of stable reference genes for qRT-PCR in Stropharia rugosoannulata using mRNA-sequencing data.PloS one · 2025Article
- Selection and validation of reference genes for RT-qPCR in ophiocordyceps sinensis under different experimental conditions.PloS one · 2024Article
- GastronOmics: Edibility and safety of mycelium of the oyster mushroomCurrent research in food science · 2024Article
- Development of reference genes for RT-qPCR analysis of gene expression in Pleurotus pulmonarius for biotechnological applications.Scientific reports · 2023Article
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- Establishment of an Efficient Polyethylene Glycol (PEG)-Mediated Transformation System inJournal of fungi (Basel, Switzerland) · 2022Article
- Genome-wide identification and expression analyses of C2H2 zinc finger transcription factors inPeerJ · 2022Article
- Effect of Nutritional Factors and Copper on the Regulation of Laccase Enzyme Production inJournal of fungi (Basel, Switzerland) · 2021Article
- Strain Degeneration inJournal of fungi (Basel, Switzerland) · 2021Article
- Trends and Applications of Omics Technologies to Functional Characterisation of Enzymes and Protein Metabolites Produced by Fungi.Journal of fungi (Basel, Switzerland) · 2021Review
- Selection and validation of endogenous reference genes for RT-qPCR normalization in different stresses and tissues of the tiger milk mushroom,Mycoscience · 2021Article
- Selection and Validation of Reference Genes for RT-qPCR Normalization inFrontiers in physiology · 2021Article
- Comparative Proteomic Analysis ofInternational journal of molecular sciences · 2019Article
- Article
- Reference Gene Expression in Adipose-Derived Stromal Cells Undergoing Adipogenic Differentiation.Tissue engineering. Part C, Methods · 2019Article
- Selection of Reference Genes for qRT-PCR Analysis inMolecules (Basel, Switzerland) · 2018Article
- Systematic Analysis of the Pleurotus ostreatus Laccase Gene (PoLac) Family and Functional Characterization of PoLac2 Involved in the Degradation of Cotton-Straw Lignin.Molecules (Basel, Switzerland) · 2018Article
- Selection and Evaluation of Appropriate Reference Genes for RT-qPCR Normalization ofBioMed research international · 2018Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recently, the lignin-degrading basidiomycete Pleurotus ostreatus has become a widely used model organism for fungal genomic and transcriptomic analyses. The increasing interest in this species has led to an increasing number of studies analyzing the transcriptional regulation of multigene families that encode extracellular enzymes. Reverse transcription (RT) followed by real-time PCR is the most suitable technique for analyzing the expression of gene sets under multiple culture conditions. In this work, we tested the suitability of 13 candidate genes for their use as reference genes in P. ostreatus time course cultures for enzyme production. We applied three different statistical algorithms and obtained a combination of stable reference genes for optimal normalization of RT-quantitative PCR assays. This reference index can be used for future transcriptomic analyses and validation of transcriptome sequencing or microarray data. Moreover, we analyzed the expression patterns of a laccase and a manganese peroxidase (lacc10 and mnp3, respectively) in lignocellulose and glucose-based media using submerged, semisolid, and solid-state fermentation. By testing different normalization strategies, we demonstrate that the use of nonvalidated reference genes as internal controls leads to biased results and misinterpretations of the biological responses underlying expression changes.
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