Evidence map›Paper›PMID 25744406›Full record

ArticleLife sciences2015

Inhibition of microglial activation by elderberry extracts and its phenolic components.

Agnes Simonyi, Zihong Chen, Jinghua Jiang, Yijia Zong, Dennis Y Chuang, Zezong Gu, Chi-Hua Lu, Kevin L Fritsche, C Michael Greenlief, George E Rottinghaus and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Life sciences, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed
1.7field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed, 53 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Effect of Elderberry (Nutrients · 2024
    Article
  5. GenusInternational journal of molecular sciences · 2024
    Review
  6. Article
  7. Article
  8. Article
  9. Review
  10. Article
  11. Review
  12. Article
  13. Article
  14. Review
  15. Review
  16. Elderberry (Foods (Basel, Switzerland) · 2019
    Article
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 1 institution in 1 country.

Agnes SimonyiMU Center for Botanical Interaction Studies, University of Missouri, Columbia, MO, USA; Department of Biochemistry, University of Missouri, Columbia, MO, USA; Interdisciplinary Neuroscience Program, University of Missouri, Columbia, MO, USA; Center for Translational Neuroscience, University of Missouri, Columbia, MO, USA.
Zihong ChenMU Center for Botanical Interaction Studies, University of Missouri, Columbia, MO, USA; Department of Biochemistry, University of Missouri, Columbia, MO, USA.
Jinghua JiangMU Center for Botanical Interaction Studies, University of Missouri, Columbia, MO, USA; Department of Biochemistry, University of Missouri, Columbia, MO, USA.
Yijia ZongMU Center for Botanical Interaction Studies, University of Missouri, Columbia, MO, USA; Interdisciplinary Neuroscience Program, University of Missouri, Columbia, MO, USA; Center for Translational Neuroscience, University of Missouri, Columbia, MO, USA.
Dennis Y ChuangMU Center for Botanical Interaction Studies, University of Missouri, Columbia, MO, USA; Interdisciplinary Neuroscience Program, University of Missouri, Columbia, MO, USA; Center for Translational Neuroscience, University of Missouri, Columbia, MO, USA.
Zezong GuMU Center for Botanical Interaction Studies, University of Missouri, Columbia, MO, USA; Interdisciplinary Neuroscience Program, University of Missouri, Columbia, MO, USA; Center for Translational Neuroscience, University of Missouri, Columbia, MO, USA; Department of Pathology and Anatomical Sciences, University of Missouri, Columbia, MO, USA.
Chi-Hua LuMU Center for Botanical Interaction Studies, University of Missouri, Columbia, MO, USA; Department of Animal Sciences, University of Missouri, Columbia, MO, USA.
Kevin L FritscheMU Center for Botanical Interaction Studies, University of Missouri, Columbia, MO, USA; Department of Animal Sciences, University of Missouri, Columbia, MO, USA.
C Michael GreenliefMU Center for Botanical Interaction Studies, University of Missouri, Columbia, MO, USA; Department of Chemistry, University of Missouri, Columbia, MO, USA.
George E RottinghausMU Center for Botanical Interaction Studies, University of Missouri, Columbia, MO, USA; Veterinary Medical Diagnostic Laboratory, University of Missouri, Columbia, MO, USA.
Andrew L ThomasMU Center for Botanical Interaction Studies, University of Missouri, Columbia, MO, USA; University of Missouri Southwest Research Center, Mt. Vernon, MO, USA.
Dennis B LubahnMU Center for Botanical Interaction Studies, University of Missouri, Columbia, MO, USA; Department of Biochemistry, University of Missouri, Columbia, MO, USA; Department of Animal Sciences, University of Missouri, Columbia, MO, USA.
Grace Y SunMU Center for Botanical Interaction Studies, University of Missouri, Columbia, MO, USA; Department of Biochemistry, University of Missouri, Columbia, MO, USA; Interdisciplinary Neuroscience Program, University of Missouri, Columbia, MO, USA; Center for Translational Neuroscience, University of Missouri, Columbia, MO, USA; Department of Pathology and Anatomical Sciences, University of Missouri, Columbia, MO, USA. Electronic address: sung@missouri.edu.
University of Missouri · US

Funding

PATHOGENESIS OF PHOSPHOLIPASES A2 IN ADP01AG018357 · NIA · UNIVERSITY OF MISSOURI-COLUMBIA · PI SUN, GRACE Y · 2001 to 2012
$10.4M
The Interactions Core/Mark McintoshP50AT006273 · NCCIH · UNIVERSITY OF MISSOURI-COLUMBIA · PI LUBAHN, DENNIS B · 2010 to 2014
$7.4M
NCCIH NIH HHS 1P50 AT006273NCCIH NIH HHS P50 AT006273NIA NIH HHS 2P01 AG08357NIA NIH HHS P01 AG018357
6 · The paper itself

Abstract

aimsElderberry (Sambucus spp.) is one of the oldest medicinal plants noted for its cardiovascular, anti-inflammatory, and immune-stimulatory properties. In this study, we investigated the anti-inflammatory and anti-oxidant effects of the American elderberry (Sambucus nigra subsp. canadensis) pomace as well as some of the anthocyanins (cyanidin chloride and cyanidin 3-O-glucoside) and flavonols (quercetin and rutin) in bv-2 mouse microglial cells. MAIN

methodsThe bv-2 cells were pretreated with elderberry pomace (extracted with ethanol or ethyl acetate) or its anthocyanins and flavonols and stimulated by either lipopolysaccharide (LPS) or interferon-γ (IFNγ). Reactive oxygen species (ROS) and nitric oxide (NO) production (indicating oxidative stress and inflammatory response) were measured using the ROS detection reagent DCF-DA and the Griess reaction, respectively. KEY

findingsAnalysis of total monomeric anthocyanin (as cyanidin 3-O-glucoside equivalents) indicated five-fold higher amount in the freeze-dried ethanol extract as compared to that of the oven-dried extract; anthocyanin was not detected in the ethyl acetate extracts. Elderberry ethanol extracts (freeze-dried or oven-dried) showed higher anti-oxidant activities and better ability to inhibit LPS or IFNγ-induced NO production as compared with the ethyl acetate extracts. The phenolic compounds strongly inhibited LPS or IFNγ-induced ROS production, but except for quercetin, they were relatively poor in inhibiting NO production. SIGNIFICANCE: These results demonstrated differences in anti-oxidative and anti-inflammatory effects of elderberry extracts depending on solvents used. Results further identified quercetin as the most active component in suppressing oxidative stress and inflammatory responses on microglial cells.

Indexed as

AnimalsAnthocyaninsCells, CulturedCell SurvivalLipopolysaccharidesMiceMicrogliaNitric OxidePhenolsPlant ExtractsQuercetinReactive Oxygen SpeciesSambucusAnthocyaninsLipopolysaccharidesNitric OxidePhenolsPlant ExtractsQuercetinReactive Oxygen SpeciesElderberryLipopolysaccharideMicrogliaNitric oxideQuercetinReactive oxygen species

Identifiers

PMID25744406
PMCPMC4405485
OpenAlexW1972308802

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.