ArticleBiochimica et biophysica acta2015
Human FABP1 T94A variant enhances cholesterol uptake.
Article in Biochimica et biophysica acta, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- What frog gill resorption brings: loss of function, cell death, and metabolic reorganization.Frontiers in zoology · 2024Article
- Effects of genetic selection and threonine on meat quality in Pekin ducks.Poultry science · 2020Article
- ΔJournal of lipid research · 2018Article
- Label-free Proteomic Analysis of Exosomes Derived from Inducible Hepatitis B Virus-Replicating HepAD38 Cell Line.Molecular & cellular proteomics : MCP · 2017Article
- Impact of dietary phytol on lipid metabolism in SCP2/SCPX/L-FABP null mice.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2017Article
- Review
- Relative contributions of L-FABP, SCP-2/SCP-x, or both to hepatic biliary phenotype of female mice.Archives of biochemistry and biophysics · 2015Article
- Impact of SCP-2/SCP-x gene ablation and dietary cholesterol on hepatic lipid accumulation.American journal of physiology. Gastrointestinal and liver physiology · 2015Article
- Loss of L-FABP, SCP-2/SCP-x, or both induces hepatic lipid accumulation in female mice.Archives of biochemistry and biophysics · 2015Article
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Authors and funding
10 authors.
Funding
Abstract
Although expression of the human liver fatty acid binding protein (FABP1) T94A variant alters serum lipoprotein cholesterol levels in human subjects, nothing is known whereby the variant elicits these effects. This issue was addressed by in vitro cholesterol binding assays using purified recombinant wild-type (WT) FABP1 T94T and T94A variant proteins and in cultured primary human hepatocytes expressing the FABP1 T94T (genotyped as TT) or T94A (genotyped as CC) proteins. The human FABP1 T94A variant protein had 3-fold higher cholesterol-binding affinity than the WT FABP1 T94T as shown by NBD-cholesterol fluorescence binding assays and by cholesterol isothermal titration microcalorimetry (ITC) binding assays. CC variant hepatocytes also exhibited 30% higher total FABP1 protein. HDL- and LDL-mediated NBD-cholesterol uptake was faster in CC variant than TT WT human hepatocytes. VLDL-mediated uptake of NBD-cholesterol did not differ between CC and TT human hepatocytes. The increased HDL- and LDL-mediated NBD-cholesterol uptake was not associated with any significant change in mRNA levels of SCARB1, LDLR, CETP, and LCAT encoding the key proteins in lipoprotein cholesterol uptake. Thus, the increased HDL- and LDL-mediated NBD-cholesterol uptake by CC hepatocytes may be associated with higher affinity of T94A protein for cholesterol and/or increased total T94A protein level.
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