ArticleJournal of experimental botany2015
The identification of new cytosolic glutamine synthetase and asparagine synthetase genes in barley (Hordeum vulgare L.), and their expression during leaf senescence.
Article in Journal of experimental botany, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 94 citations in OpenAlex.
- Enhanced Activity of Glutamine Synthetase by Manipulating a GWAS-Identified BnaA02.GLN1;2 Gene Augments Nitrate Uptake and Yield in Brassica napus.Plant, cell & environment · 2026Article
- Coordinated regulation of nitrogen transport and assimilation drives high nitrogen use efficiency in sugarcane.Frontiers in plant science · 2026Article
- Regulation of HvASN1 expression by bZIP transcription factors during barley embryo development and germination.Planta · 2025Article
- The Role of Glutamine Synthetase (GS) and Glutamate Synthase (GOGAT) in the Improvement of Nitrogen Use Efficiency in Cereals.Biomolecules · 2023Review
- Multilayered regulation of developmentally programmed pre-anthesis tip degeneration of the barley inflorescence.The Plant cell · 2023Article
- Uncovering genomic and transcriptional variations facilitates utilization of wild resources in cotton disease resistance improvement.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Magnesium promotes tea plant growth via enhanced glutamine synthetase-mediated nitrogen assimilation.Plant physiology · 2023Article
- Variability and limits of nitrogen and phosphorus resorption during foliar senescence.Plant communications · 2023Review
- Transcriptome Analysis Reveals the Genes Related to Water-Melon Fruit Expansion under Low-Light Stress.Plants (Basel, Switzerland) · 2023Article
- Understanding the Relationships between Free Asparagine in Grain and Other Traits to Breed Low-Asparagine Wheat.Plants (Basel, Switzerland) · 2022Review
- The Evolution of Leaf Function during Development Is Reflected in Profound Changes in the Metabolic Composition of the Vacuole.Metabolites · 2021Article
- Genotypic Variation of Nitrogen Use Efficiency and Amino Acid Metabolism in Barley.Frontiers in plant science · 2021Article
- Identification of Differentially Expressed Genes and Pathways Involved in Growth and Development ofFrontiers in plant science · 2021Article
- Dark-Induced Barley Leaf Senescence - A Crop System for Studying Senescence and Autophagy Mechanisms.Frontiers in plant science · 2021Review
- Cereal asparagine synthetase genes.The Annals of applied biology · 2021Article
- Seasonal senescence of leaves and roots of Populus trichocarpa-is the scenario the same or different?Tree physiology · 2020Article
- Global transcriptome analysis of alfalfa reveals six key biological processes of senescent leaves.PeerJ · 2020Article
- Manipulating Amino Acid Metabolism to Improve Crop Nitrogen Use Efficiency for a Sustainable Agriculture.Frontiers in plant science · 2020Review
- Asparagine Synthesis During Tobacco Leaf Curing.Plants (Basel, Switzerland) · 2019Article
- Acrylamide in food: Progress in and prospects for genetic and agronomic solutions.The Annals of applied biology · 2019Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Glutamine synthetase and asparagine synthetase are two master enzymes involved in ammonium assimilation in plants. Their roles in nitrogen remobilization and nitrogen use efficiency have been proposed. In this report, the genes coding for the cytosolic glutamine synthetases (HvGS1) and asparagine synthetases (HvASN) in barley were identified. In addition to the three HvGS1 and two HvASN sequences previously reported, two prokaryotic-like HvGS1 and three HvASN cDNA sequences were identified. Gene structures were then characterized, obtaining full genomic sequences. The response of the five HvGS1 and five HvASN genes to leaf senescence was then studied. Developmental senescence was studied using primary and flag leaves. Dark-exposure or low-nitrate conditions were also used to trigger stress-induced senescence. Well-known senescence markers such as the chlorophyll and Rubisco contents were monitored in order to characterize senescence levels in the different leaves. The three eukaryotic-like HvGS1_1, HvGS1_2, and HvGS1_3 sequences showed the typical senescence-induced reduction in gene expression described in many plant species. By contrast, the two prokaryotic-like HvGS1_4 and HvGS1_5 sequences were repressed by leaf senescence, similar to the HvGS2 gene, which encodes the chloroplast glutamine synthetase isoenzyme. There was a greater contrast in the responses of the five HvASN and this suggested that these genes are needed for N remobilization in senescing leaves only when plants are well fertilized with nitrate. Responses of the HvASN sequences to dark-induced senescence showed that there are two categories of asparagine synthetases, one induced in the dark and the other repressed by the same conditions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.