ArticleJournal of industrial microbiology & biotechnology2015
Efficient expression of nattokinase in Bacillus licheniformis: host strain construction and signal peptide optimization.
Article in Journal of industrial microbiology & biotechnology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 84 citations in OpenAlex.
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- Engineering the Tat-secretion pathway of Bacillus licheniformis for the secretion of cytoplasmic enzyme arginase.Applied microbiology and biotechnology · 2024Article
- Understanding energy fluctuation during the transition state: The role of AbrB in Bacillus licheniformis.Microbial cell factories · 2024Article
- Computational analysis to comprehend the structure-function properties of fibrinolytic enzymes fromHeliyon · 2024Article
- Identification of a Novel Chitinase fromFoods (Basel, Switzerland) · 2024Article
- Efficient expression of γ-glutamyl transpeptidase in Bacillus subtilis via CRISPR/Cas9n and its immobilization.Applied microbiology and biotechnology · 2024Article
- Research progress on the fibrinolytic enzymes produced from traditional fermented foods.Food science & nutrition · 2023Review
- Enhancement of alkaline protease production in recombinant Bacillus licheniformis by response surface methodology.Bioresources and bioprocessing · 2023Article
- The potential role of ischaemia-reperfusion injury in chronic, relapsing diseases such as rheumatoid arthritis, Long COVID, and ME/CFS: evidence, mechanisms, and therapeutic implications.The Biochemical journal · 2022Review
- Biotechnology, Bioengineering and Applications ofBiomolecules · 2022Review
- Recent Advances in Nattokinase-Enriched Fermented Soybean Foods: A Review.Foods (Basel, Switzerland) · 2022Review
- Enhanced production of poly-γ-glutamic acid via optimizing the expression cassette ofSynthetic and systems biotechnology · 2022Article
- Enhanced extracellular α-amylase production in Brevibacillus choshinensis by optimizing extracellular degradation and folding environment.Journal of industrial microbiology & biotechnology · 2022Article
- Construction Method of Industrial College in Vocational Colleges Based on Cluster Analysis Algorithm.Computational and mathematical methods in medicine · 2022Article
- Tofu processing wastewater as a low-cost substrate for high activity nattokinase production using Bacillus subtilis.BMC biotechnology · 2021Article
- Heterologous expression of nattokinase from B. subtilis natto using Pichia pastoris GS115 and assessment of its thrombolytic activity.BMC biotechnology · 2021Article
- Food-grade expression of nattokinase in Lactobacillus delbrueckii subsp. bulgaricus and its thrombolytic activity in vitro.Biotechnology letters · 2020Article
- Enhanced production of heterologous proteins via engineering the cell surface of Bacillus licheniformis.Journal of industrial microbiology & biotechnology · 2019Article
- Transcriptional Changes in the Xylose Operon inInternational journal of molecular sciences · 2019Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nattokinase (NK) possesses the potential for prevention and treatment of thrombus-related diseases. In this study, high-level expression of nattokinase was achieved in Bacillus licheniformis WX-02 via host strain construction and signal peptides optimization. First, ten genes (mpr, vpr, aprX, epr, bpr, wprA, aprE, bprA, hag, amyl) encoding for eight extracellular proteases, a flagellin and an amylase were deleted to obtain B. licheniformis BL10, which showed no extracellular proteases activity in gelatin zymography. Second, the gene fragments of P43 promoter, Svpr, nattokinase and TamyL were combined into pHY300PLK to form the expression vector pP43SNT. In BL10 (pP43SNT), the fermentation activity and product activity per unit of biomass of nattokinase reached 14.33 FU/mL and 2,187.71 FU/g respectively, which increased by 39 and 156 % compared to WX-02 (pP43SNT). Last, Svpr was replaced with SsacC and SbprA, and the maximum fermentation activity (33.83 FU/mL) was achieved using SsacC, which was 229 % higher than that of WX-02 (pP43SNT). The maximum NK fermentation activity in this study reaches the commercial production level of solid state fermentation, and this study provides a promising engineered strain for industrial production of nattokinase, as well as a potential platform host for expression of other target proteins.
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