Evidence map›Paper›PMID 25399061›Full record

ArticleEuropean journal of nutrition2015

Differences in micronucleus frequency and acrylamide adduct levels with hemoglobin between vegetarians and non-vegetarians.

Natalia Kotova, Cecilia Frostne, Lilianne Abramsson-Zetterberg, Eden Tareke, Rolf Bergman, Siamak Haghdoost, Birgit Paulsson, Margareta Törnqvist, Dan Segerbäck, Dag Jenssen and 1 more

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Article in European journal of nutrition, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
5.0field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 26 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 1 country.

Natalia KotovaDepartment of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, 106 91, Stockholm, Sweden. Natalia.Kotova@su.se.
Cecilia FrostneEnvironmental Chemistry Unit, Department of Materials and Environmental Chemistry, Stockholm University, 106 91, Stockholm, Sweden.
Lilianne Abramsson-ZetterbergDepartment of Science, The Swedish National Food Agency, Box 622, 751 26, Uppsala, Sweden.
Eden TarekeEnvironmental Chemistry Unit, Department of Materials and Environmental Chemistry, Stockholm University, 106 91, Stockholm, Sweden.
Rolf Bergman, Knivsbrunna, 755 98, Uppsala, Sweden.
Siamak HaghdoostDepartment of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, 106 91, Stockholm, Sweden.
Birgit PaulssonEnvironmental Chemistry Unit, Department of Materials and Environmental Chemistry, Stockholm University, 106 91, Stockholm, Sweden.
Margareta TörnqvistEnvironmental Chemistry Unit, Department of Materials and Environmental Chemistry, Stockholm University, 106 91, Stockholm, Sweden.
Dan SegerbäckDepartment of Biosciences and Nutrition, Karolinska Institute, 141 83, Huddinge, Sweden.
Dag JenssenDepartment of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, 106 91, Stockholm, Sweden.
Jan GrawéThe Rudbeck Laboratory, Cell Analysis Core Facility, Uppsala University, 751 85, Uppsala, Sweden.
Stockholm University · SEKarolinska Institutet · SENational Food Administration · SEScience for Life Laboratory · SE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeNutrients and food constituents can prevent or contribute to genotoxicity. In this study, the possible influence of a vegetarian/non-vegetarian diet on genotoxic effects was investigated in 58 non-smoking healthy vegetarians (V) and non-vegetarians (NV), age 21-37 years from the Stockholm area in Sweden.

methodsPhysical activity and dietary habits were similar in both groups, with the exception of the intake of meat and fish. Using flow cytometry, we determined the formation of micronuclei (MN) in transferrin-positive immature peripheral blood reticulocytes (Trf-Ret) (Total: n = 53; V: n = 27; NV: n = 26). Dietary exposure to acrylamide was measured through hemoglobin (Hb) adducts in peripheral erythrocytes (Total: n = 53; V: n = 29; NV: n = 24). Hb adducts of both acrylamide and its genotoxic metabolite glycidamide were monitored as a measure of the corresponding in vivo doses.

resultsOur data demonstrated that compared with the non-vegetarians, the vegetarians exhibited lower frequencies of MN (fMN) in the Trf-Ret (p < 0.01, Student's t test). A multivariate analysis demonstrated that there was no association between the fMN and factors such as age, sex, intake of vitamins/minerals, serum folic acid and vitamin B12 levels, physical activity, and body mass index. The mean Hb adduct levels of acrylamide and glycidamide showed no significant differences between vegetarians and non-vegetarians. Furthermore, there were no significant relationships between the adduct levels and fMN in the individuals. The ratio of the Hb adduct levels from glycidamide and acrylamide, however, showed a significant difference (p < 0.04) between the two groups.

conclusionsThese data suggest that the vegetarian diet might be beneficial in lowering genomic instability in healthy individuals. The measured Hb adduct levels indicate that the total intake of acrylamide does not differ between the two studied groups and does not contribute to the observed difference in fMN, although an influence of the diet on the metabolic rates of acrylamide was indicated. In addition, the observed significant difference in the background fMN in the two groups demonstrated that the MN analysis method has a sensitivity applicable to the biomonitoring of human lifestyle factors.

Indexed as

Feeding BehaviorMicronucleus TestsVegetariansAcrylamideAdultBody Mass IndexDiet, VegetarianDNA DamageEpoxy CompoundsErythrocytesFemaleFolic AcidGenomic InstabilityHemoglobinsHumansLife StyleAcrylamideEpoxy CompoundsFolic AcidglycidamideHemoglobinsTransferrinVitamin B 12BiomonitoringHemoglobin adductsMicronucleiNon-vegetarianVegetarian

Identifiers

PMID25399061
OpenAlexW1969938865

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.