ArticlePlanta2015
Gene amplification of 5-enol-pyruvylshikimate-3-phosphate synthase in glyphosate-resistant Kochia scoparia.
Article in Planta, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 130 citations in OpenAlex.
- Target-site and non-target-site mechanisms confer multiple herbicide resistance in waterhemp (Amaranthus tuberculatus) accessions from Wisconsin.Pest management science · 2026Article
- FHY3/FAR1 transposable elements generate adaptive genetic variation in the Bassia scoparia genome.Pest management science · 2025Article
- Resistance to Amino Acid Biosynthesis Inhibiting-Herbicides inPlants (Basel, Switzerland) · 2025Article
- MurA-catalyzed synthesis of 5-enolpyruvylshikimate-3-phosphate confers glyphosate tolerance in bryophytes.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- A Study on Genetically Engineered Foods: Need, Benefits, Risk, and Current Knowledge.Cell biochemistry and biophysics · 2024Review
- Article
- Genomic-based epidemiology reveals independent origins and gene flow of glyphosate resistance in Bassia scoparia populations across North America.Molecular ecology · 2021Article
- Effect of EPSPS gene copy number and glyphosate selection on fitness of glyphosate-resistant Bassia scoparia in the field.Scientific reports · 2021Article
- Evolution of Glyphosate-Resistant Weeds.Reviews of environmental contamination and toxicology · 2021Review
- Fitness Cost Associated With EnhancedFrontiers in plant science · 2021Article
- High gene flow maintains genetic diversity following selection for high EPSPS copy number in the weed kochia (Amaranthaceae).Scientific reports · 2020Article
- Multiple mutations in the EPSPS and ALS genes of Amaranthus hybridus underlie resistance to glyphosate and ALS inhibitors.Scientific reports · 2020Article
- Mechanisms of evolved herbicide resistance.The Journal of biological chemistry · 2020Review
- Physiological performance of glyphosate and imazamox mixtures on Amaranthus palmeri sensitive and resistant to glyphosate.Scientific reports · 2019Article
- The Draft Genome of Kochia scoparia and the Mechanism of Glyphosate Resistance via Transposon-Mediated EPSPS Tandem Gene Duplication.Genome biology and evolution · 2019Article
- Weed resistance to synthetic auxin herbicides.Pest management science · 2018Review
- Population Genetic Structure in Glyphosate-Resistant and -Susceptible Palmer Amaranth (Frontiers in plant science · 2018Article
- Pro-106-Ser mutation and EPSPS overexpression acting together simultaneously in glyphosate-resistant goosegrass (Eleusine indica).Scientific reports · 2017Article
- Selection of relatively exact reference genes for gene expression studies in goosegrass (Eleusine indica) under herbicide stress.Scientific reports · 2017Article
- Physical Mapping of Amplified Copies of the 5-Enolpyruvylshikimate-3-Phosphate Synthase Gene in Glyphosate-Resistant Amaranthus tuberculatus.Plant physiology · 2017Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MAIN
conclusionField-evolved resistance to the herbicide glyphosate is due to amplification of one of two EPSPS alleles, increasing transcription and protein with no splice variants or effects on other pathway genes. The widely used herbicide glyphosate inhibits the shikimate pathway enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS). Globally, the intensive use of glyphosate for weed control has selected for glyphosate resistance in 31 weed species. Populations of suspected glyphosate-resistant Kochia scoparia were collected from fields located in the US central Great Plains. Glyphosate dose response verified glyphosate resistance in nine populations. The mechanism of resistance to glyphosate was investigated using targeted sequencing, quantitative PCR, immunoblotting, and whole transcriptome de novo sequencing to characterize the sequence and expression of EPSPS. Sequence analysis showed no mutation of the EPSPS Pro106 codon in glyphosate-resistant K. scoparia, whereas EPSPS genomic copy number and transcript abundance were elevated three- to ten-fold in resistant individuals relative to susceptible individuals. Glyphosate-resistant individuals with increased relative EPSPS copy numbers had consistently lower shikimate accumulation in leaf disks treated with 100 μM glyphosate and EPSPS protein levels were higher in glyphosate-resistant individuals with increased gene copy number compared to glyphosate-susceptible individuals. RNA sequence analysis revealed seven nucleotide positions with two different expressed alleles in glyphosate-susceptible reads. However, one nucleotide at the seven positions was predominant in glyphosate-resistant sequences, suggesting that only one of two EPSPS alleles was amplified in glyphosate-resistant individuals. No alternatively spliced EPSPS transcripts were detected. Expression of five other genes in the chorismate pathway was unaffected in glyphosate-resistant individuals with increased EPSPS expression. These results indicate increased EPSPS expression is a mechanism for glyphosate resistance in these K. scoparia populations.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.