ArticleClinical nutrition (Edinburgh, Scotland)2014
New stable isotope method to measure protein digestibility and response to pancreatic enzyme intake in cystic fibrosis.
Article in Clinical nutrition (Edinburgh, Scotland), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT01494909 (Development Of An Innovative Panel of Methods To Measure Intestinal Macronutrient Digestion, Absorption, and Function), which is not on this map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Development Of An Innovative Panel of Methods To Measure Intestinal Macronutrient Digestion, Absorption, and Function
Who cites it
25 citing papers in PubMed, 53 citations in OpenAlex.
- Differences in amino acid digestibility between young and older adults: a randomized crossover study using the dual tracer method.The American journal of clinical nutrition · 2026Trial
- Investigating the Digestibility, Bioavailability and Utilization of Protein Blends in Older Adults Using a Dual Stable Isotope Tracer Technique.Nutrients · 2025Trial
- Trial
- Blood 15N:13C Enrichment Ratios Are Proportional to the Ingested Quantity of Protein with the Dual-Tracer Approach for Determining Amino Acid Bioavailability in Humans.The Journal of nutrition · 2020Trial
- True ileal digestibility of legumes determined by dual-isotope tracer method in Indian adults.The American journal of clinical nutrition · 2019Trial
- Metabolic and molecular responses to leucine-enriched branched chain amino acid supplementation in the skeletal muscle of alcoholic cirrhosis.Hepatology (Baltimore, Md.) · 2015Trial
- Advances in Nutritional and Therapeutic Strategies for Type 2 Intestinal Failure: A Narrative Review.Nutrition reviews · 2025Review
- Validation of dietary whey protein substrate challenge absorption test as an indicator of proteolysis.PloS one · 2025Article
- Nasogastric bolus administration of a protein-rich drink augments insulinaemia and aminoacidaemia but not whole-body protein turnover or muscle protein synthesis versus oral administration.Clinical science (London, England : 1979) · 2024Article
- Evaluation of Protein Quality in Humans and Insights on Stable Isotope Approaches to Measure Digestibility - A Review.Advances in nutrition (Bethesda, Md.) · 2022Review
- Utilization of the Healthy Eating Index in Cystic Fibrosis.Nutrients · 2022Article
- Protein and amino acid digestibility ofEuropean journal of nutrition · 2021Article
- Intestinal function is impaired in patients with Chronic Obstructive Pulmonary Disease.Clinical nutrition (Edinburgh, Scotland) · 2021Observational
- Walking exercise alters protein digestion, amino acid absorption, and whole body protein kinetics in older adults with and without COPD.Journal of applied physiology (Bethesda, Md. : 1985) · 2021Article
- Developmental changes in the utilization of citrulline by neonatal pigs.American journal of physiology. Renal physiology · 2020Article
- Quantifying the contribution of dietary protein to whole body protein kinetics: examination of the intrinsically labeled proteins method.American journal of physiology. Endocrinology and metabolism · 2019Review
- Protein quality & amino acid requirements in relation to needs in India.The Indian journal of medical research · 2018Review
- Nutritional Requirements of Lung Transplant Recipients: Challenges and Considerations.Nutrients · 2018Review
- Measurement of protein digestibility in humans by a dual-tracer method.The American journal of clinical nutrition · 2018Article
- Personalizing protein nourishment.Critical reviews in food science and nutrition · 2017Review
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
BACKGROUND &
aimsAdequate protein intake and digestion are necessary to prevent muscle wasting in cystic fibrosis (CF). Accurate and easy-to-use methodology to quantify protein maldigestion is lacking in CF.
objectiveTo measure protein digestibility and the response to pancreatic enzyme intake in CF by using a new stable isotope methodology.
designIn 19 CF and 8 healthy subjects, protein digestibility was quantified during continuous (sip) feeding for 6 h by adding (15)N-labeled spirulina protein and L-[ring-(2)H5]phenylalanine (PHE) to the nutrition and measuring plasma ratio [(15)N]PHE to [(2)H5]PHE. Pancreatic enzymes were ingested after 2 h in CF and the response in protein digestibility was assessed. To exclude difference in mucosal function, postabsorptive whole-body citrulline (CIT) production rate was measured by L-[5-(13)C-5,5-(2)H2]-CIT pulse and blood samples were taken to analyze tracer-tracee ratios.
resultsProtein digestibility was severely reduced in the CF group (47% of healthy subjects; P < 0.001). Intake of pancreatic enzymes induced a slow increase in protein digestibility in CF until 90% of values obtained by healthy subjects. Maximal digestibility was reached at 100 min and maintained for 80 min. Stratification into CF children (n = 10) and adults showed comparable values for protein digestibility and similar kinetic responses to pancreatic enzyme intake. Whole-body citrulline production was elevated in CF indicating preserved mucosal function.
conclusionProtein digestibility is severely compromised in patients with CF as measured by this novel and easy-to-use stable isotope approach. Pancreatic enzymes are able to normalize protein digestibility in CF, albeit with a severe delay. Registration ClinicalTrials.gov = NCT01494909.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.