ArticleLipids2013
EPA and DHA levels in whole blood decrease more rapidly when stored at -20 °C as compared with room temperature, 4 and -75 °C.
Article in Lipids, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 74 citations in OpenAlex.
- Differences in the effects of school meals on children's cognitive performance according to gender, household education and baseline reading skills.European journal of clinical nutrition · 2016Trial
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- Global variations in omega-3 fatty acid status and omega-6:omega-3 ratios: insights from > 500,000 whole-blood dried blood spot samples.Lipids in health and disease · 2025Article
- Investigation into temporal changes in the human bloodstain lipidome.International journal of legal medicine · 2025Article
- Lipidomic and Fatty Acid Biomarkers in Whole Blood Can Predict the Dietary Intake of Eicosapentaenoic and Docosahexaenoic Acids in a Danish Population.The Journal of nutrition · 2024Article
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- The Relationship between Ozone and Human Blood in the Course of a Well-Controlled, Mild, and Transitory Oxidative Eustress.Antioxidants (Basel, Switzerland) · 2021Article
- From bedside to bench-practical considerations to avoid pre-analytical pitfalls and assess sample quality for high-resolution metabolomics and lipidomics analyses of body fluids.Analytical and bioanalytical chemistry · 2021Review
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- Whole-blood PUFA and associations with markers of nutritional and health status in acutely malnourished children in Cambodia.Public health nutrition · 2020Article
- Fatty Acid Profile of Postmenopausal Women Receiving, and Not Receiving, Hormone Replacement Therapy.International journal of environmental research and public health · 2019Article
- A review on advances in methods for modification of paper supports for use in point-of-care testing.Mikrochimica acta · 2019Review
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- Best practices for the design, laboratory analysis, and reporting of trials involving fatty acids.The American journal of clinical nutrition · 2018Article
- Physical activity level among children recovering from severe acute malnutrition.Tropical medicine & international health : TM & IH · 2018Article
- Incorporation of Dairy Lipids in the Diet Increased Long-Chain Omega-3 Fatty Acids Status in Post-weaning Rats.Frontiers in nutrition · 2018Article
- Correlates of whole-blood polyunsaturated fatty acids among young children with moderate acute malnutrition.Nutrition journal · 2017Article
- Comparing identified and statistically significant lipids and polar metabolites in 15-year old serum and dried blood spot samples for longitudinal studies.Rapid communications in mass spectrometry : RCM · 2017Article
- Data on the phospholipid fatty acyl composition of retroperitoneal white adipose tissue in ad libitum fed and fasted mice.Data in brief · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High-throughput n-3 fatty acid profiling is enabled by collection techniques such as venous whole blood and fingertip prick (FTP) sampling, but the resulting increased sample numbers increases storage demand. Highly unsaturated fatty acids (HUFA) in erythrocytes are susceptible to oxidation, but this tendency is poorly characterized in venous and FTP whole blood. Presently, whole blood samples with low and high n-3 content collected with ethylenediaminetetraacetic acid were stored on chromatography paper with and without BHT pre-treatment for up to 180 days at different temperatures (room, 4, -20, -75 °C). Whole blood prepared with heparin and BHT and stored in cryovials was also examined. Eicosapentaenoic acid (EPA, 20:5n-3) + docosahexaenoic acid (DHA, 22:6n-3) is relatively stable when stored at -75 °C under various conditions but rapidly decreases in whole blood when stored at -20 °C. At -20 °C, BHT + heparin prepared whole blood can prevent decreases in cryovials up to 180 days but BHT only slows the decreases on chromatography paper. Surprisingly, whole blood stored at 4 °C and room temperature was less susceptible to decreases in EPA + DHA as compared with -20 °C storage. Assessments of n-3 blood biomarkers indicate the % n-3 HUFA in total HUFA was more stable as compared with the sum of the relative % of EPA + DHA. In conclusion, FTP and venous whole blood for fatty acid analysis should be stored at -75 °C whenever possible. In the absence of -75 °C storage conditions, BHT should be added and 4 °C or room temperature appear to be better alternatives to -20 °C.
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