ArticleScientific reports2013
Improved identification of wheat gluten proteins through alkylation of cysteine residues and peptide-based mass spectrometry.
Article in Scientific reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed.
- Characterization among and within Sicilian Tetraploid Wheat Landraces by Gluten Protein Analysis for Traceability Purposes.Plants (Basel, Switzerland) · 2024Article
- Proteomic Characterization of Wheat Protein Fractions Taken at Different Baking Conditions.Journal of agricultural and food chemistry · 2023Article
- Post-translational modifications and glycoprofiling of palivizumab by UHPLC-RPLC/HILIC and mass spectrometry.Journal of proteins and proteomics · 2022Article
- Post translational modifications of milk proteins in geographically diverse goat breeds.Scientific reports · 2021Article
- Comprehensive Comparison of Clinically Relevant Grain Proteins in Modern and Traditional Bread Wheat Cultivars.International journal of molecular sciences · 2020Article
- Quantitation of the immunodominant 33-mer peptide from α-gliadin in wheat flours by liquid chromatography tandem mass spectrometry.Scientific reports · 2017Article
- Isolation and characterization of gluten protein types from wheat, rye, barley and oats for use as reference materials.PloS one · 2017Article
- Formation and reshuffling of disulfide bonds in bovine serum albumin demonstrated using tandem mass spectrometry with collision-induced and electron-transfer dissociation.Scientific reports · 2015Article
- ProPepper: a curated database for identification and analysis of peptide and immune-responsive epitope composition of cereal grain protein families.Database : the journal of biological databases and curation · 2015Article
- Intrinsic Size Parameters for Palmitoylated and Carboxyamidomethylated Peptides.International journal of mass spectrometry · 2014Article
- Article
- Gluten Detection and Speciation by Liquid Chromatography Mass Spectrometry (LC-MS/MS).Foods (Basel, Switzerland) · 2013Article
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5 authors.
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Abstract
The concentration and composition of wheat gluten proteins and the presence, concentration and location of cysteine residues therein are important for wheat flour quality. However, it is difficult to identify gluten proteins, as they are an extremely polymorphic mixture of prolamins. We here present methods for cysteine labeling of wheat prolamins with 4-vinylpyridine (4-VP) and iodoacetamide (IDAM) which, as compared to label-free analysis, substantially improve identification of cysteine-containing peptides in enzymic prolamin digests by electrospray ionization--tandem mass spectrometry. Both chymotrypsin and thermolysin yielded cysteine-containing peptides from different gluten proteins, but more proteins could be identified after chymotryptic digestion. In addition, to the best of our knowledge, we were the first to label prolamins with isotope coded affinity tags (ICAT), which are commonly used for quantitative proteomics. However, more peptides were detected after labeling gluten proteins with 4-VP and IDAM than with ICAT.
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