ArticleJournal of pharmacy & bioallied sciences2013
Thermal stability of high concentration lysozyme across varying pH: A Fourier Transform Infrared study.
Article in Journal of pharmacy & bioallied sciences, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 127 citations in OpenAlex.
- Performance comparison of downstream unit operations and process chains for the purification of amino acids for food, feed and pharma applications.Biotechnology reports (Amsterdam, Netherlands) · 2026Review
- The Effects of pH on Orientations of Adsorbed Lysozyme on Negatively Charged Surfaces.The journal of physical chemistry. B · 2026Article
- Development and Optimization of Ionic Strength-Responsive Lipid-Polymer Hybrid Nanoparticles for Buccal Protein Delivery.Pharmaceutics · 2026Article
- Comparison of Protein Binding and Fluorescence Quenching by Gold and Iron Oxide Nanoparticles: pH and Temperature Dependence.Molecules (Basel, Switzerland) · 2026Article
- A new type of biomarker for heat stress: insights from immunology.Frontiers in immunology · 2026Review
- The Thermodynamic and Gelation Properties of Ovalbumin and Lysozyme.Gels (Basel, Switzerland) · 2025Article
- A novel virus potentially evolved from the N4-like viruses represents a unique viral family:Applied and environmental microbiology · 2024Article
- Sound-mediated nucleation and growth of amyloid fibrils.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Antimicrobial Properties of Colostrum and Milk.Antibiotics (Basel, Switzerland) · 2024Review
- Nanocrystallites Modulate Intermolecular Interactions in Cryoprotected Protein Solutions.The journal of physical chemistry. B · 2023Article
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- Interaction of Hyaluronan Acid with Some Proteins in Aqueous Solution as Studied by NMR.Membranes · 2023Article
- Tear nanoDSF Denaturation Profile Is Predictive of Glaucoma.International journal of molecular sciences · 2023Article
- Penicillin Binding Protein 7/8 Is a Potential Drug Target in Carbapenem-Resistant Acinetobacter baumannii.Antimicrobial agents and chemotherapy · 2023Article
- Differential fibril morphologies and thermostability determine functional roles ofFrontiers in molecular biosciences · 2023Article
- Conformational Consequences for Compatible Osmolytes on Thermal Denaturation.Life (Basel, Switzerland) · 2021Article
- UV Resonance Raman explores protein structural modification upon fibrillation and ligand interaction.Biophysical journal · 2021Article
- Amyloid Self-Assembly of Lysozyme in Self-Crowded Conditions: The Formation of a Protein Oligomer Hydrogel.Biomacromolecules · 2021Article
- Minoxidil-Coated Lysozyme-Shelled Microbubbes Combined With Ultrasound for the Enhancement of Hair Follicle Growth: EfficacyFrontiers in pharmacology · 2021Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimThe current work is aimed at understanding the effect of pH on the thermal stability of hen egg white lysozyme (HEWL) at high concentration (200 mg/mL). MATERIALS AND
methodsFourier Transform Infrared (FTIR) Spectroscopy with modified hardware and software to overcome some of the traditional challenges like water subtraction, sample evaporation, proper purging etc., are used in this study.
resultsHEWL was subjected to thermal stress at pH 3.0-7.0 between 25°C and 95°C and monitored by FTIR spectroscopy. Calculated Tm values showed that the enzyme exhibited maximum thermal stability at pH 5.0. Second derivative plots constructed in the amide I region suggested that at pH 5.0 the enzyme possessed higher amount of α-helix and lower amount of aggregates, when compared to other pHs.
conclusionsConsidering the fact that HEWL has attractive applications in various industries and being processed under different experimental conditions including high temperatures, our work is able to reveal the reason behind the pH dependent thermal stability of HEWL at high concentration, when subjected to heat denaturation. In future, studies should aim at using various excipients that may help to increase the stability and activity of the enzyme at this high concentration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.