ArticleApplied and environmental microbiology2013
Characterization of a wild, novel nisin a-producing Lactococcus strain with an L. lactis subsp. cremoris genotype and an L. lactis subsp. lactis phenotype, isolated from Greek raw milk.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 56 citations in OpenAlex.
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- Effect of Probiotic Bacteria on the Gut Microbiome of Mice with Lipopolysaccharide-Induced Inflammation.Microorganisms · 2024Article
- Antilisterial activity of raw sheep milk from two native Epirus breeds: Culture-dependent identification, bacteriocin gene detection and primary safety evaluation of the antagonistic LAB biota.Current research in microbial sciences · 2024Article
- Lactococcus lactis, a bacterium with probiotic functions and pathogenicity.World journal of microbiology & biotechnology · 2023Review
- Lactococcus lactis endocarditis and liver abscess in an immunocompetent patient: a case report and review of the literature.Journal of medical case reports · 2023Review
- Bioengineering of a Lactococcus lactis subsp. lactis strain enhances nisin production and bioactivity.PloS one · 2023Article
- Impact of an Omega-3-Enriched Sheep Diet on the Microbiota and Chemical Composition of Kefalograviera Cheese.Foods (Basel, Switzerland) · 2022Article
- A novel bacteriocin isolated fromFrontiers in nutrition · 2022Article
- Safety Evaluation, Biogenic Amine Formation, and Enzymatic Activity Profiles of Autochthonous Enterocin-Producing Greek Cheese Isolates of theMicroorganisms · 2021Article
- Modulating effects of the probioticExperimental and therapeutic medicine · 2021Article
- Cell wall polysaccharides of Gram positive ovococcoid bacteria and their role as bacteriophage receptors.Computational and structural biotechnology journal · 2021Review
- Role of Starter Cultures on the Safety of Fermented Meat Products.Frontiers in microbiology · 2019Review
- Hurdle factors minimizing growth ofAIMS microbiology · 2018Article
- A review on Lactococcus lactis: from food to factory.Microbial cell factories · 2017Review
- Evaluation of Lactococcus lactis Isolates from Nondairy Sources with Potential Dairy Applications Reveals Extensive Phenotype-Genotype Disparity and Implications for a Revised Species.Applied and environmental microbiology · 2015Article
- Purification and Characterization of Bacteriocin Produced by Weissella confusa A3 of Dairy Origin.PloS one · 2015Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Several molecular taxonomic studies have revealed that many natural (wild) Lactococcus lactis strains of dairy origin which are phenotypically representative of the L. lactis subspecies lactis cluster genotypically within subspecies cremoris and vice versa. Recently, we isolated two wild nisin-producing (Nis(+)) L. lactis strains, M78 and M104, of the lactis phenotype from Greek raw milk (J. Samelis, A. Lianou, A. Kakouri, C. Delbès, I. Rogelj, B. B. Matijašic, and M. C. Montel, J. Food Prot. 72:783-790, 2009); strain M78 possess a novel nisin A sequence (GenBank accession number HM219853). In this study, the actual subspecies identity of M78 and M104 isolates was elucidated, using 16S rRNA and acmA (encoding lactococcal N-acetylmuramidase) gene and histidine biosynthesis operon polymorphisms and 16S rRNA and ldh (encoding lactate dehydrogenase) gene phylogenies. Except the acmA gene analysis, molecular tools revealed that isolates M78 and M104 clustered with strains of the cremoris genotype, including the LMG 6897(T) strain, while they were distant from strains of the lactis genotype, including the LMG 6890(T) strain. The two wild isolates had identical repetitive sequence-based PCR (rep-PCR), randomly amplified polymorphic DNA (RAPD), plasmid, and whole-cell protein profiles and shared high 16S rRNA (99.9%) and ldh (100%) gene sequence homologies. In contrast, they exhibited identical sugar fermentation and enzymatic patterns which were similar to those of the subspecies lactis LMG 6890(T) strain. To our knowledge, this is the first complete identification report on a wild L. lactis subsp. cremoris genotype of the lactis phenotype which is capable of nisin A production and, thus, has strong potential for use as a novel dairy starter and/or protective culture.
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