ArticleAnnals of clinical microbiology and antimicrobials2012
Use of tuf as a target for sequence-based identification of Gram-positive cocci of the genus Enterococcus, Streptococcus, coagulase-negative Staphylococcus, and Lactococcus.
Article in Annals of clinical microbiology and antimicrobials, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Molecular Characterization of Methicillin and Aminoglycoside-Resistant Staphylococcus Species from Drinking Water and Clinical Samples in Dutsin-Ma, Northwestern Nigeria.Journal of epidemiology and global health · 2026Article
- Decolorization of methyl orange and aniline red dyes by Enterococcus hirae isolated from beach sand.Scientific reports · 2025Article
- Nanopore-based amplicon sequencing for rapid detection and identification ofFrontiers in microbiology · 2025Article
- Genus-targeted markers for the taxonomic identification and monitoring of coagulase-positive and coagulase-negative Staphylococcus species.World journal of microbiology & biotechnology · 2024Article
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- Determination of the Prevalence and Antimicrobial Resistance ofAntibiotics (Basel, Switzerland) · 2023Article
- A Comprehensive Study on Antibiotic Resistance among Coagulase-Negative Staphylococci (CoNS) Strains Isolated from Ready-to-Eat Food Served in Bars and Restaurants.Foods (Basel, Switzerland) · 2023Article
- Molecular Analysis of Pathogenicity, Adhesive Matrix Molecules (MSCRAMMs) and Biofilm Genes of Coagulase-Negative Staphylococci Isolated from Ready-to-Eat Food.International journal of environmental research and public health · 2023Article
- Isolation and characterization of bacteriophages from wastewater sources onIranian journal of microbiology · 2021Article
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- Identification of Clinically RelevantPathogens (Basel, Switzerland) · 2020Article
- Diagnosis of human and canineHeliyon · 2020Article
- Epidemiological analysis of Trueperella abortisuis isolated from cases of pig abortion of a single farm.Folia microbiologica · 2020Article
- Investigation of SCCmec types I-IV in clinical isolates of methicillin-resistant coagulase-negative staphylococci in Ahvaz, Southwest Iran.Bioscience reports · 2020Article
- Underrated Staphylococcus species and their role in antimicrobial resistance spreading.Genetics and molecular biology · 2020Article
- Comparison of 16S Ribosomal RNA Targeted Sequencing and Culture for Bacterial Identification in Normally Sterile Body Fluid Samples: Report of a 10-Year Clinical Laboratory Review.Annals of laboratory medicine · 2020Article
- Development of a reference data set for assigningAntimicrobial resistance and infection control · 2019Article
- The Diverse Functional Roles of Elongation Factor Tu (EF-Tu) in Microbial Pathogenesis.Frontiers in microbiology · 2019Review
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAccurate identification of isolates belonging to genus Enterococcus, Streptococcus, coagulase-negative Staphylococcus, and Lactococcus at the species level is necessary to provide a better understanding of their pathogenic potential, to aid in making clinical decisions, and to conduct epidemiologic investigations,especially when large blind samples must be analyzed. It is useful to simultaneously identify species in different genera using a single primer pair.
methodsWe developed a primer pair based on the tuf gene (encoding elongation factor) sequence to identify 56 Gram-positive cocci isolates.
resultsThe target sequences were amplified from all 56 samples. The sequencing results and the phylogenetic tree derived from the partial tuf gene sequences identified the isolates as three enterococcal species, two lactococcal species, two staphylococcal species, and six streptococcal species, as well as eight isolates that were novel species of the genus Streptococcus. Partial gene sequence analysis of the sodA, dnaK, and 16S RNA genes confirmed the results obtained by tuf gene sequencing.
conclusionBased on the uniform amplification of the tuf gene from all samples and the ability to identify all isolates at both the genus and species levels, we conclude that the primer pair developed in this research provides a powerful tool for identifying these organisms in clinical laboratories where large blind samples are used.
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