ArticlePloS one2012
Plant 45S rDNA clusters are fragile sites and their instability is associated with epigenetic alterations.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Methylcytosine oxidation derivatives are preferentially associated with repetitive chromatin and mark active rDNA inFrontiers in plant science · 2026Article
- Research Progress on 35S rDNA and 5S rDNA in Sugarcane: Challenges and Prospects.International journal of molecular sciences · 2025Review
- Whole-transcriptome sequencing reveals the global molecular responses and ceRNA regulatory network involved in programmed cell death of rice cultivars zyk639 and zyk-lm.BMC genomics · 2025Article
- Chromatin dynamics and RNA metabolism are double-edged swords for the maintenance of plant genome integrity.Nature plants · 2024Review
- Genomic and cytogenetic analyses reveal satellite repeat signature in allotetraploid okra (Abelmoschus esculentus).BMC plant biology · 2024Article
- Investigating the Origin and Evolution of PolyploidPlants (Basel, Switzerland) · 2023Article
- Migration of repetitive DNAs during evolution of the permanent translocation heterozygosity in the oyster plant (Tradescantia section Rhoeo).Chromosoma · 2022Article
- Identification of 45S rDNA in Passiflora using low coverage sequencing: analysis of GC content and chromosomal localization.Molecular biology reports · 2022Article
- Authentication ofPlants (Basel, Switzerland) · 2022Article
- Article
- Upon heat stress processing of ribosomal RNA precursors into mature rRNAs is compromised after cleavage at primary P site inRNA biology · 2022Article
- A Cross between Bread Wheat and a 2D(2R) Disomic Substitution Triticale Line Leads to the Formation of a Novel Disomic Addition Line and Provides Information of the Role of Rye Secalins on Breadmaking Characteristics.International journal of molecular sciences · 2020Article
- Comparative Chromosomal Localization of 45S and 5S rDNA Sites in 76 Purple-Fleshed Sweet Potato Cultivars.Plants (Basel, Switzerland) · 2020Article
- Chromatin Remodeling Protein ZmCHB101 Regulates Nitrate-Responsive Gene Expression in Maize.Frontiers in plant science · 2020Article
- H3K9 Demethylation-Induced R-Loop Accumulation Is Linked to Disorganized Nucleoli.Frontiers in genetics · 2020Article
- Dynamic changes in histone modification are associated with upregulation of Hsf and rRNA genes during heat stress in maize seedlings.Protoplasma · 2019Article
- DetectingInternational journal of molecular sciences · 2019Article
- Relationship between epigenetic marks and the behavior of 45S rDNA sites in chromosomes and interphase nuclei of Lolium-Festuca complex.Molecular biology reports · 2018Article
- Karyotype reshufflings of Festuca pratensis × Lolium perenne hybrids.Protoplasma · 2018Article
- Karyotype heterogeneity in Philodendron s.l. (Araceae) revealed by chromosome mapping of rDNA loci.PloS one · 2018Article
Corrections and comments
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Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Our previous study demonstrated that 45S ribosomal DNA (45S rDNA) clusters were chromosome fragile sites expressed spontaneously in Lolium. In this study, fragile phenotypes of 45S rDNA were observed under aphidicolin (APH) incubation in several plant species. Further actinomycin D (ActD) treatment showed that transcriptional stress might interfere with chromatin packaging, resulting in 45S rDNA fragile expression. These data identified 45S rDNA sites as replication-dependent as well as transcription-dependent fragile sites in plants. In the presence of ActD, a dramatic switch to an open chromatin conformation and accumulated incomplete 5' end of the external transcribed spacer (5'ETS) transcripts were observed, accompanied by decreased DNA methylation, decreased levels of histone H3, and increased histone acetylation and levels of H3K4me2, suggesting that these epigenetic alterations are associated with failure of 45S rDNA condensation. Furthermore, the finding that γ-H2AX was accumulated at 45S rDNA sites following ActD treatment suggested that the DNA damage signaling pathway was associated with the appearance of 45S rDNA fragile phenotypes. Our data provide a link between 45S rDNA transcription and chromatin-packaging defects and open the door for further identifying the molecular mechanism involved.
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