ArticlePloS one2012
A novel strategy to construct yeast Saccharomyces cerevisiae strains for very high gravity fermentation.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Dynamic, model-guided intensification of fuel ethanol production in yeast: proteome constraints, CRISPR rewiring, and high-gravity fermentations.Archives of microbiology · 2026Review
- The potential for Scotch Malt Whisky flavour diversification by yeast.FEMS yeast research · 2024Review
- Review
- Screening ester-producing yeasts to fortify the brewing of rice-flavor Baijiu for enhanced aromas.Bioengineered · 2023Article
- Increasing Solvent Tolerance to Improve Microbial Production of Alcohols, Terpenoids and Aromatics.Microorganisms · 2021Review
- Construction of advanced producers of first- and second-generation ethanol in Saccharomyces cerevisiae and selected species of non-conventional yeasts (Scheffersomyces stipitis, Ogataea polymorpha).Journal of industrial microbiology & biotechnology · 2020Review
- Improvement ofFood science and biotechnology · 2018Article
- Overexpression of smORF YNR034W-A/EGO4 in Saccharomyces cerevisiae increases the fermentative efficiency of Agave tequilana Weber must.Journal of industrial microbiology & biotechnology · 2017Article
- Evidence for a Role for the Plasma Membrane in the Nanomechanical Properties of the Cell Wall as Revealed by an Atomic Force Microscopy Study of the Response of Saccharomyces cerevisiae to Ethanol Stress.Applied and environmental microbiology · 2016Article
- Enhanced ethanol production via electrostatically accelerated fermentation of glucose using Saccharomyces cerevisiae.Scientific reports · 2015Article
- Genomic reconstruction to improve bioethanol and ergosterol production of industrial yeast Saccharomyces cerevisiae.Journal of industrial microbiology & biotechnology · 2015Article
- Modifying Yeast Tolerance to Inhibitory Conditions of Ethanol Production Processes.Frontiers in bioengineering and biotechnology · 2015Review
- Large-scale robot-assisted genome shuffling yields industrial Saccharomyces cerevisiae yeasts with increased ethanol tolerance.Biotechnology for biofuels · 2015Article
- Stress Tolerance Variations in Saccharomyces cerevisiae Strains from Diverse Ecological Sources and Geographical Locations.PloS one · 2015Article
- Improving industrial yeast strains: exploiting natural and artificial diversity.FEMS microbiology reviews · 2014Review
- A system based network approach to ethanol tolerance in Saccharomyces cerevisiae.BMC systems biology · 2014Article
- The relationship between lysine 4 on histone H3 methylation levels of alcohol tolerance genes and changes of ethanol tolerance in Saccharomyces cerevisiae.Microbial biotechnology · 2014Article
- Streptomycin resistance-aided genome shuffling to improve doramectin productivity of Streptomyces avermitilis NEAU1069.Journal of industrial microbiology & biotechnology · 2013Article
- Construction of novel Saccharomyces cerevisiae strains for bioethanol active dry yeast (ADY) production.PloS one · 2013Article
- Genome sequencing and genetic breeding of a bioethanol Saccharomyces cerevisiae strain YJS329.BMC genomics · 2012Article
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9 authors.
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Abstract
Very high gravity (VHG) fermentation is aimed to considerably increase both the fermentation rate and the ethanol concentration, thereby reducing capital costs and the risk of bacterial contamination. This process results in critical issues, such as adverse stress factors (ie., osmotic pressure and ethanol inhibition) and high concentrations of metabolic byproducts which are difficult to overcome by a single breeding method. In the present paper, a novel strategy that combines metabolic engineering and genome shuffling to circumvent these limitations and improve the bioethanol production performance of Saccharomyces cerevisiae strains under VHG conditions was developed. First, in strain Z5, which performed better than other widely used industrial strains, the gene GPD2 encoding glycerol 3-phosphate dehydrogenase was deleted, resulting in a mutant (Z5ΔGPD2) with a lower glycerol yield and poor ethanol productivity. Second, strain Z5ΔGPD2 was subjected to three rounds of genome shuffling to improve its VHG fermentation performance, and the best performing strain SZ3-1 was obtained. Results showed that strain SZ3-1 not only produced less glycerol, but also increased the ethanol yield by up to 8% compared with the parent strain Z5. Further analysis suggested that the improved ethanol yield in strain SZ3-1 was mainly contributed by the enhanced ethanol tolerance of the strain. The differences in ethanol tolerance between strains Z5 and SZ3-1 were closely associated with the cell membrane fatty acid compositions and intracellular trehalose concentrations. Finally, genome rearrangements in the optimized strain were confirmed by karyotype analysis. Hence, a combination of genome shuffling and metabolic engineering is an efficient approach for the rapid improvement of yeast strains for desirable industrial phenotypes.
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