ArticleMolecular biotechnology2012
Lactobacillus plantarum and Lactobacillus buchneri as expression systems: evaluation of different origins of replication for the design of suitable shuttle vectors.
Article in Molecular biotechnology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 38 citations in OpenAlex.
- Harnessing endogenous CRISPR-Cas9 for inducible genetic engineering ofApplied and environmental microbiology · 2026Article
- An optimized CRISPR/Cas9-based genome editing platform enhances bacteriocin production in Lactiplantibacillus plantarum.Journal of biological engineering · 2026Article
- Advances in Genetic Transformation of Lactic Acid Bacteria: Overcoming Barriers and Enhancing Plasmid Tools.International journal of molecular sciences · 2025Review
- Commensal acidification of specific gut regions produces a protective priority effect against enteropathogenic bacterial infection.Applied and environmental microbiology · 2025Article
- Construction and evaluation of recombinant Lactobacillus plantarum expressing Micropterus salmoides hepcidin.Microbial cell factories · 2025Article
- The insertion of the inverted repeat of an insertion sequence (IS) element fromApplied and environmental microbiology · 2025Article
- A symbiotic physical niche in Drosophila melanogaster regulates stable association of a multi-species gut microbiota.Nature communications · 2023Article
- In vitro assembly of plasmid DNA for direct cloning in Lactiplantibacillus plantarum WCSF1.PloS one · 2023Article
- Construction and Application of a Plasmid-Based Signal Peptide Library for Improved Secretion of Recombinant Proteins withMicroorganisms · 2022Article
- Recent progress on n-butanol production by lactic acid bacteria.World journal of microbiology & biotechnology · 2021Review
- Conjugative DNA Transfer FromFrontiers in microbiology · 2021Article
- A novel universal nano-luciferase-involved reporter system for long-term probing food-borne probiotics and pathogenic bacteria in mice byRSC advances · 2020Article
- Article
- Broad range shuttle vector construction and promoter evaluation for the use ofSynthetic biology (Oxford, England) · 2019Article
- Evaluation of novel inducible promoter/repressor systems for recombinant protein expression in Lactobacillus plantarum.Microbial cell factories · 2016Article
- Tuning constitutive recombinant gene expression in Lactobacillus plantarum.Microbial cell factories · 2014Article
- Biochemical characterization of the major N-acetylmuramidase from Lactobacillus buchneri.Microbiology (Reading, England) · 2014Article
- L-Arabinose isomerase and D-xylose isomerase from Lactobacillus reuteri: characterization, coexpression in the food grade host Lactobacillus plantarum, and application in the conversion of D-galactose and D-glucose.Journal of agricultural and food chemistry · 2014Article
- Protein O-glucosylation in Lactobacillus buchneri.Glycoconjugate journal · 2014Article
- "Direct cloning in Lactobacillus plantarum: electroporation with non-methylated plasmid DNA enhances transformation efficiency and makes shuttle vectors obsolete".Microbial cell factories · 2012Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The objectives of this study were to establish transformation protocols for Lactobacillus plantarum CD033 and Lactobacillus buchneri CD034, two industrial silage strains and to test the influence of selected origins of replication on plasmid copy number, plasmid stability, and plasmid incompatibility in these strains. Electro-transformation protocols were optimized by examination of the influence of different electroporation solutions and cell wall weakening agents on transformation efficiency. Using Lithium acetate as cell wall weakening agent, we could achieve transformation efficiencies of 8 × 10(4) transformants per 1 μg DNA for L. buchneri CD034 which is to our knowledge the highest described for this species up to now. In order to test feasibility of previously described origins of replication derived from Bacillus subtilis, L. plantarum, Lactococcus lactis, and two novel L. buchneri CD034 plasmids to drive replication in our two selected Lactobacillus strains, six shuttle vectors were constructed. Results indicate that, in terms of stable propagation and high gene copy numbers (up to 238 copies/chromosome), the most suitable origins of replication for the construction of expression vectors for the selected silage strains were the ones derived from the novel L. buchneri CD034 plasmids.
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