ArticleApplied and environmental microbiology2011
Investigating antibacterial effects of garlic (Allium sativum) concentrate and garlic-derived organosulfur compounds on Campylobacter jejuni by using Fourier transform infrared spectroscopy, Raman spectroscopy, and electron microscopy.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers, 1 of them a synthesis that pooled it.
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Who cites it
54 citing papers in PubMed, 1 synthesis or guideline pooled it, 147 citations in OpenAlex.
- Herbal Medicine for the Management of Wounds: A Systematic Review of Clinical Studies.Infectious disorders drug targets · 2025Pooled it
- Spectroscopic Techniques in Bacterial Analysis: Applications of FTIR and Raman-Review.Foods (Basel, Switzerland) · 2026Review
- Development of surface enhanced Raman spectra coupled with machine learning analysis for differentiation of closely related species within Enterobacter cloacae complex.BMC microbiology · 2026Article
- Recent Research Advances in Multi-Functional Diallyl Trisulfide (DATS): A Comprehensive Review of Characteristics, Metabolism, Pharmacodynamics, Applications, and Drug Delivery Systems.International journal of nanomedicine · 2025Review
- Exploring the antimicrobial potential of crude peptide extracts fromPharmaceutical biology · 2024Article
- Review
- Evaluation of bioactivity and antibacterial properties of Ti6Al4V-based green biocomposite implant encompassing TiOHeliyon · 2024Article
- Rapid discrimination of four Salmonella enterica serovars: A performance comparison between benchtop and handheld Raman spectrometers.Journal of cellular and molecular medicine · 2024Article
- Differentiation of closely-related species within Acinetobacter baumannii-calcoaceticus complex via Raman spectroscopy: a comparative machine learning analysis.World journal of microbiology & biotechnology · 2024Article
- Formulation of Garlic Essential Oil-assisted Silver Nanoparticles and Mechanistic Evaluation of their Antimicrobial Activity against a Spectrum of Pathogenic Microorganisms.Current topics in medicinal chemistry · 2024Article
- Antibacterial activity and mechanisms of α-terpineol against foodborne pathogenic bacteria.Applied microbiology and biotechnology · 2023Article
- Biodegradation Capabilities of Paraquat-Degrading Bacteria Immobilized on Nanoceramics.Toxics · 2023Article
- Ability of Garlic and Ginger Oil to ReduceAnimals : an open access journal from MDPI · 2022Article
- Advancements in Skin Delivery of Natural Bioactive Products for Wound Management: A Brief Review of Two Decades.Pharmaceutics · 2022Review
- Article
- Allicin in Digestive System Cancer: From Biological Effects to Clinical Treatment.Frontiers in pharmacology · 2022Review
- Preparation and Optimization of Garlic Oil/Apple Cider Vinegar Nanoemulsion Loaded with Minoxidil to Treat Alopecia.Pharmaceutics · 2021Article
- Korean traditional foods as antiviral and respiratory disease prevention and treatments: A detailed review.Trends in food science & technology · 2021Review
- Campylobacter jejuni Antimicrobial Resistance Profiles and Mechanisms Determined Using a Raman Spectroscopy-Based Metabolomic Approach.Applied and environmental microbiology · 2021Article
- Antimicrobial Activity of Sorghum Phenolic Extract on Bovine Foodborne and Mastitis-Causing Pathogens.Antibiotics (Basel, Switzerland) · 2021Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fourier transform infrared (FT-IR) spectroscopy and Raman spectroscopy were used to study the cell injury and inactivation of Campylobacter jejuni from exposure to antioxidants from garlic. C. jejuni was treated with various concentrations of garlic concentrate and garlic-derived organosulfur compounds in growth media and saline at 4, 22, and 35°C. The antimicrobial activities of the diallyl sulfides increased with the number of sulfur atoms (diallyl sulfide < diallyl disulfide < diallyl trisulfide). FT-IR spectroscopy confirmed that organosulfur compounds are responsible for the substantial antimicrobial activity of garlic, much greater than those of garlic phenolic compounds, as indicated by changes in the spectral features of proteins, lipids, and polysaccharides in the bacterial cell membranes. Confocal Raman microscopy (532-nm-gold-particle substrate) and Raman mapping of a single bacterium confirmed the intracellular uptake of sulfur and phenolic components. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were employed to verify cell damage. Principal-component analysis (PCA), discriminant function analysis (DFA), and soft independent modeling of class analogs (SIMCA) were performed, and results were cross validated to differentiate bacteria based upon the degree of cell injury. Partial least-squares regression (PLSR) was employed to quantify and predict actual numbers of healthy and injured bacterial cells remaining following treatment. PLSR-based loading plots were investigated to further verify the changes in the cell membrane of C. jejuni treated with organosulfur compounds. We demonstrated that bacterial injury and inactivation could be accurately investigated by complementary infrared and Raman spectroscopies using a chemical-based, "whole-organism fingerprint" with the aid of chemometrics and electron microscopy.
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Registered trials
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