ArticlePloS one2010
Proteome-wide search reveals unexpected RNA-binding proteins in Saccharomyces cerevisiae.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 96 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
96 citing papers in PubMed, 163 citations in OpenAlex.
- RGG-motif protein Scd6 affects oxidative stress response by regulating cytosolic caTalase T1 (Ctt1).RNA biology · 2026Article
- Uukuniemi virus infection causes a pervasive remodelling of the RNA-binding proteome in tick cells.PLoS pathogens · 2025Article
- A highly sensitive protein-RNA cross-linking mass spectrometry workflow with enhanced structural modeling potential.Nucleic acids research · 2025Article
- A genetic screen identifiesmicroPublication biology · 2024Article
- Moonlighting Proteins: Diverse Functions Found in Fungi.Journal of fungi (Basel, Switzerland) · 2023Review
- Parallel laboratory evolution and rational debugging reveal genomic plasticity toCell genomics · 2023Article
- Investigating the Prevalence of RNA-Binding Metabolic Enzymes inInternational journal of molecular sciences · 2023Article
- RNA-dependent interactome allows network-based assignment of RNA-binding protein function.Nucleic acids research · 2023Article
- Surveying the global landscape of post-transcriptional regulators.Nature structural & molecular biology · 2023Article
- Small noncoding RNA interactome capture reveals pervasive, carbon source-dependent tRNA engagement of yeast glycolytic enzymes.RNA (New York, N.Y.) · 2023Article
- The mutual interaction of glycolytic enzymes and RNA in post-transcriptional regulation.RNA (New York, N.Y.) · 2022Review
- Spliceosomal SL1 RNA binding to U1-70K: the role of the extended RRM.Nucleic acids research · 2022Article
- Review
- RNA Binding Proteins as Pioneer Determinants of Infection: Protective, Proviral, or Both?Viruses · 2021Review
- Analysis of the TORC1 interactome reveals a spatially distinct function of TORC1 in mRNP complexes.The Journal of cell biology · 2021Article
- Newly identified genes contribute to vanillin tolerance in Saccharomyces cerevisiae.Microbial biotechnology · 2021Article
- Identification of new proteins related with cisplatin resistance in Saccharomyces cerevisiae.Applied microbiology and biotechnology · 2021Article
- RNA-Centric Approaches to Profile the RNA-Protein Interaction Landscape on Selected RNAs.Non-coding RNA · 2021Review
- Functional Roles of RNA-Binding Proteins in Plant Signaling.Life (Basel, Switzerland) · 2020Review
- Elucidation of the RNA-granule inducing sodium azide stress response through transcriptome analysis.Genomics · 2020Article
36 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The vast landscape of RNA-protein interactions at the heart of post-transcriptional regulation remains largely unexplored. Indeed it is likely that, even in yeast, a substantial fraction of the regulatory RNA-binding proteins (RBPs) remain to be discovered. Systematic experimental methods can play a key role in discovering these RBPs--most of the known yeast RBPs lack RNA-binding domains that might enable this activity to be predicted. We describe here a proteome-wide approach to identify RNA-protein interactions based on in vitro binding of RNA samples to yeast protein microarrays that represent over 80% of the yeast proteome. We used this procedure to screen for novel RBPs and RNA-protein interactions. A complementary mass spectrometry technique also identified proteins that associate with yeast mRNAs. Both the protein microarray and mass spectrometry methods successfully identify previously annotated RBPs, suggesting that other proteins identified in these assays might be novel RBPs. Of 35 putative novel RBPs identified by either or both of these methods, 12, including 75% of the eight most highly-ranked candidates, reproducibly associated with specific cellular RNAs. Surprisingly, most of the 12 newly discovered RBPs were enzymes. Functional characteristics of the RNA targets of some of the novel RBPs suggest coordinated post-transcriptional regulation of subunits of protein complexes and a possible link between mRNA trafficking and vesicle transport. Our results suggest that many more RBPs still remain to be identified and provide a set of candidates for further investigation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.