ArticleJournal of bacteriology2010
Elucidation of beta-oxidation pathways in Ralstonia eutropha H16 by examination of global gene expression.
Article in Journal of bacteriology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 116 citations in OpenAlex.
- Advancements in genetic engineering for enhanced Polyhydroxyalkanoates (PHA) production: a comprehensive review of metabolic pathway manipulation and gene deletion strategies.Bioengineered · 2025Review
- Retainment of Poly(3-hydroxybutyrate-Polymers · 2025Article
- Insights into genetic determinants of volatile fatty acid catabolism inApplied and environmental microbiology · 2025Article
- Carbon monoxide-oxidising Pseudomonadota on volcanic deposits.Environmental microbiome · 2025Article
- Exploring Fatty Acid β-Oxidation Pathways in Bacteria: From General Mechanisms to DSF Signaling and Pathogenicity in Xanthomonas.Current microbiology · 2024Review
- Synthetic biology toolkit of Ralstonia eutropha (Cupriavidus necator).Applied microbiology and biotechnology · 2024Review
- Biosynthesis of Polyhydroxyalkanoates inPolymers · 2024Article
- Medium-Chain-Length Fatty Acid Catabolism in Cupriavidus necator H16: Transcriptome Sequencing Reveals Differences from Long-Chain-Length Fatty Acid β-Oxidation and Involvement of Several Homologous Genes.Applied and environmental microbiology · 2023Article
- A genome-scale metabolic model of Cupriavidus necator H16 integrated with TraDIS and transcriptomic data reveals metabolic insights for biotechnological applications.PLoS computational biology · 2022Article
- Impact of various β-ketothiolase genes on PHBHHx production in Cupriavidus necator H16 derivatives.Applied microbiology and biotechnology · 2022Article
- Insights into the Degradation of Medium-Chain-Length Dicarboxylic Acids in Cupriavidus necator H16 Reveal β-Oxidation Differences between Dicarboxylic Acids and Fatty Acids.Applied and environmental microbiology · 2022Article
- Synthetic biology toolkit for engineering Cupriviadus necator H16 as a platform for COBiotechnology for biofuels · 2021Review
- Engineering Cupriavidus necator H16 for the autotrophic production of (R)-1,3-butanediol.Metabolic engineering · 2021Article
- Untargeted metabolomics analysis of Ralstonia eutropha during plant oil cultivations reveals the presence of a fucose salvage pathway.Scientific reports · 2021Article
- Phosphoglycolate salvage in a chemolithoautotroph using the Calvin cycle.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Applying Statistical Design of Experiments To Understanding the Effect of Growth Medium Components on Cupriavidus necator H16 Growth.Applied and environmental microbiology · 2020Article
- Enhanced Triacylglycerol Metabolism Contributes to Efficient Oil Utilization and High-Level Production of Salinomycin in Streptomyces albus ZD11.Applied and environmental microbiology · 2020Article
- Article
- Modification of acetoacetyl-CoA reduction step in Ralstonia eutropha for biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from structurally unrelated compounds.Microbial cell factories · 2019Article
- The genetic basis of 3-hydroxypropanoate metabolism inBiotechnology for biofuels · 2019Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ralstonia eutropha H16 is capable of growth and polyhydroxyalkanoate production on plant oils and fatty acids. However, little is known about the triacylglycerol and fatty acid degradation pathways of this bacterium. We compare whole-cell gene expression levels of R. eutropha H16 during growth and polyhydroxyalkanoate production on trioleate and fructose. Trioleate is a triacylglycerol that serves as a model for plant oils. Among the genes of note, two potential fatty acid β-oxidation operons and two putative lipase genes were shown to be upregulated in trioleate cultures. The genes of the glyoxylate bypass also exhibit increased expression during growth on trioleate. We observed that single β-oxidation operon deletion mutants of R. eutropha could grow using palm oil or crude palm kernel oil as the sole carbon source, regardless of which operon was present in the genome, but a double mutant was unable to grow under these conditions. A lipase deletion mutant did not exhibit a growth defect in emulsified oil cultures but did exhibit a phenotype in cultures containing nonemulsified oil. Mutants of the glyoxylate shunt gene for isocitrate lyase were able to grow in the presence of oils, while a malate synthase (aceB) deletion mutant grew more slowly than wild type. Gene expression under polyhydroxyalkanoate storage conditions was also examined. Many findings of this analysis confirm results from previous studies by our group and others. This work represents the first examination of global gene expression involving triacylglycerol and fatty acid catabolism genes in R. eutropha.
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