ArticleElectrophoresis2010
Optimized sample preparation of endoscopic collected pancreatic fluid for SDS-PAGE analysis.
Article in Electrophoresis, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 29 citations in OpenAlex.
- Post-translational modifications of pancreatic fluid proteins collected via the endoscopic pancreatic function test (ePFT).Journal of proteomics · 2013Trial
- In vitro antibacterial effects and mechanism of action of six constituents of Litsea cubeba essential oil against Helicobacter pylori.World journal of microbiology & biotechnology · 2026Article
- A new integrated method for tissue extracellular vesicle enrichment and proteome profiling.RSC advances · 2022Article
- Comparison of Thermal and High-Pressure Gelation of Potato Protein Isolates.Foods (Basel, Switzerland) · 2020Article
- Endoscopic Pancreas Fluid Collection: Methods and Relevance for Clinical Care and Translational Science.The American journal of gastroenterology · 2016Review
- Analysis of endoscopic pancreatic function test (ePFT)-collected pancreatic fluid proteins precipitated via ultracentrifugation.JOP : Journal of the pancreas · 2013Article
- Short Gel, Long Gradient Liquid Chromatography Tandem Mass Spectrometry to Discover Urinary Biomarkers of Chronic Pancreatitis.The open proteomics journal · 2013Article
- Inflammatory protein profiling of pancreatic cyst fluid using EUS-FNA in tandem with cytokine microarray differentiates between branch duct IPMN and inflammatory cysts.Journal of immunological methods · 2012Article
- Mass Spectrometry-Based (GeLC-MS/MS) Comparative Proteomic Analysis of Endoscopically (ePFT) Collected Pancreatic and Gastroduodenal Fluids.Clinical and translational gastroenterology · 2012Article
- Proteomic analysis (GeLC-MS/MS) of ePFT-collected pancreatic fluid in chronic pancreatitis.Journal of proteome research · 2012Article
- Mass spectrometry-based proteomics for translational research: a technical overview.The Yale journal of biology and medicine · 2012Review
- Differentiating Branch Duct and Mixed IPMN in Endoscopically Collected Pancreatic Cyst Fluid via Cytokine Analysis.Gastroenterology research and practice · 2012Article
- Difference gel electrophoresis identifies differentially expressed proteins in endoscopically collected pancreatic fluid.Electrophoresis · 2011Article
- Cytokine profiling of pancreatic fluid using the ePFT collection method in tandem with a multiplexed microarray assay.Journal of immunological methods · 2011Article
- Mass spectrometry-based proteomics of endoscopically collected pancreatic fluid in chronic pancreatitis research.Proteomics. Clinical applications · 2011Review
- Proteomic analysis of endoscopically (endoscopic pancreatic function test) collected gastroduodenal fluid using in-gel tryptic digestion followed by LC-MS/MS.Proteomics. Clinical applications · 2010Article
- Sample preparation for proteomic analysis using a GeLC-MS/MS strategy.Journal of biological methodsArticle
- Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
The standardization of methods for human body fluid protein isolation is a critical initial step for proteomic analyses aimed to discover clinically relevant biomarkers. Several caveats have hindered pancreatic fluid proteomics, including the heterogeneity of samples and protein degradation. We aim to optimize sample handling of pancreatic fluid that has been collected using a safe and effective endoscopic collection method (endoscopic pancreatic function test). Using SDS-PAGE protein profiling, we investigate (i) precipitation techniques to maximize protein extraction, (ii) auto-digestion of pancreatic fluid following prolonged exposure to a range of temperatures, (iii) effects of multiple freeze-thaw cycles on protein stability, and (iv) the utility of protease inhibitors. Our experiments revealed that TCA precipitation resulted in the most efficient extraction of protein from pancreatic fluid of the eight methods we investigated. In addition, our data reveal that although auto-digestion of proteins is prevalent at 23 and 37 degrees C, incubation on ice significantly slows such degradation. Similarly, when the sample is maintained on ice, proteolysis is minimal during multiple freeze-thaw cycles. We have also determined the addition of protease inhibitors to be assay-dependent. Our optimized sample preparation strategy can be applied to future proteomic analyses of pancreatic fluid.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.