ArticleFree radical biology & medicine2009
Immunological detection of N-formylkynurenine in oxidized proteins.
Article in Free radical biology & medicine, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 59 citations in OpenAlex.
- Anion-π interaction-induced phase separation as a prebiotic pathway to oxygenation.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Changes in Metabolomics Profiles ofInternational journal of molecular sciences · 2025Article
- Photo-Oxidation of Therapeutic Protein Formulations: From Radical Formation to Analytical Techniques.Pharmaceutics · 2021Review
- Singlet-Oxygen-Induced Phospholipase AChemistry (Weinheim an der Bergstrasse, Germany) · 2021Article
- Machine learning prediction of methionine and tryptophan photooxidation susceptibility.Molecular therapy. Methods & clinical development · 2021Article
- Comparative Study of Oxidative Structural Modifications of Unadsorbed and Adsorbed Proteins in Whey Protein Isolate-Stabilized Oil-in-Water Emulsions under the Stress of Primary and Secondary Lipid Oxidation Products.Foods (Basel, Switzerland) · 2021Article
- Oxidative Alteration of Trp-214 and Lys-199 in Human Serum Albumin Increases Binding Affinity with Phenylbutazone: A Combined Experimental and Computational Investigation.International journal of molecular sciences · 2018Article
- Laminin targeting of a peripheral nerve-highlighting peptide enables degenerated nerve visualization.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Tripping up Trp: Modification of protein tryptophan residues by reactive oxygen species, modes of detection, and biological consequences.Free radical biology & medicine · 2015Review
- Oxidation of the tryptophan 32 residue of human superoxide dismutase 1 caused by its bicarbonate-dependent peroxidase activity triggers the non-amyloid aggregation of the enzyme.The Journal of biological chemistry · 2014Article
- Oxidative stress and the HIV-infected brain proteome.Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology · 2013Review
- Hypericin-mediated photooxidative damage of α-crystallin in human lens epithelial cells.Free radical biology & medicine · 2013Article
- The specific interaction of the photosensitizer methylene blue with acetylcholinesterase provides a model system for studying the molecular consequences of photodynamic therapy.Chemico-biological interactions · 2013Article
- Human indoleamine 2,3-dioxygenase is a catalyst of physiological heme peroxidase reactions: implications for the inhibition of dioxygenase activity by hydrogen peroxide.The Journal of biological chemistry · 2013Article
- Reactive oxygen and oxidative stress: N-formyl kynurenine in photosystem II and non-photosynthetic proteins.Photosynthesis research · 2012Review
- Cardiovascular redox and ox stress proteomics.Antioxidants & redox signaling · 2012Review
- Targeted oxidation of Torpedo californica acetylcholinesterase by singlet oxygen: identification of N-formylkynurenine tryptophan derivatives within the active-site gorge of its complex with the photosensitizer methylene blue.The Biochemical journal · 2012Article
- Article
- Oxidative stress induces protein and DNA radical formation in follicular dendritic cells of the germinal center and modulates its cell death patterns in late sepsis.Free radical biology & medicine · 2011Article
- Immunological detection of N-formylkynurenine in porphyrin-mediated photooxided lens α-crystallin.Photochemistry and photobiologyArticle
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Reactions of tryptophan residues in proteins with radical and other oxidative species frequently lead to cleavage of the indole ring, modifying tryptophan residues into N-formylkynurenine (NFK) and kynurenine. Tryptophan modification has been detected in physiologically important proteins and has been associated with a number of human disease conditions. Modified residues have been identified through various combinations of proteomic analyses, tryptic digestion, HPLC, and mass spectrometry. Here we present a novel, immunological approach using polyclonal antiserum for detection of NFK. The specificity of our antiserum is confirmed using photooxidation and radical-mediated oxidation of proteins with and without tryptophan residues. The sensitivity of our antiserum is validated through detection of NFK in photooxidized myoglobin (two tryptophan residues) and in carbonate radical-oxidized human SOD1, which contains a single tryptophan residue. Analysis of photooxidized milk also shows that our antiserum can detect NFK residues in a mixture of proteins. Results from mass spectrometric analysis of photooxidized myoglobin samples corroborate the immunological data, detecting an increase in NFK content as the extent of photooxidation increases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.