ArticleUltramicroscopy2009
Monte Carlo electron-trajectory simulations in bright-field and dark-field STEM: implications for tomography of thick biological sections.
Article in Ultramicroscopy, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 56 citations in OpenAlex.
- Damage-limited resolution for X-ray and electron microscopy of organic specimens.Acta crystallographica. Section D, Structural biology · 2026Article
- Contrast by electron microscopy in thick biological specimens.Journal of microscopy · 2025Article
- Real-time visualisation of fast nanoscale processes during liquid reagent mixing by liquid cell transmission electron microscopy.Communications chemistry · 2025Article
- Ultrastructure and Nanoporosity of Human Bone Shown with Correlative On-Axis Electron and Spectroscopic Tomographies.ACS nano · 2023Article
- Non-classical crystallization of CeONanoscale · 2023Article
- Tomographic Collection of Block-Based Sparse STEM Images: Practical Implementation and Impact on the Quality of the 3D Reconstructed Volume.Materials (Basel, Switzerland) · 2019Article
- Comparison of 3D cellular imaging techniques based on scanned electron probes: Serial block face SEM vs. Axial bright-field STEM tomography.Journal of structural biology · 2018Article
- Quantification and optimization of ADF-STEM image contrast for beam-sensitive materials.Royal Society open science · 2018Article
- Biological application of Compressed Sensing Tomography in the Scanning Electron Microscope.Scientific reports · 2016Article
- Development and application of STEM for the biological sciences.Ultramicroscopy · 2012Review
- The probe profile and lateral resolution of scanning transmission electron microscopy of thick specimens.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2012Article
- Electron microscopy of specimens in liquid.Nature nanotechnology · 2011Review
- Dual-axis electron tomography of biological specimens: Extending the limits of specimen thickness with bright-field STEM imaging.Journal of structural biology · 2011Article
- Simulating STEM imaging of nanoparticles in micrometers-thick substrates.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2010Article
- Intracellular membrane traffic at high resolution.Methods in cell biology · 2010Article
- Nanoscale 3D cellular imaging by axial scanning transmission electron tomography.Nature methods · 2009Article
- Exploring photosynthesis by electron tomography.Photosynthesis researchReview
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
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Abstract
A Monte Carlo electron-trajectory calculation has been implemented to assess the optimal detector configuration for scanning transmission electron microscopy (STEM) tomography of thick biological sections. By modeling specimens containing 2 and 3 at% osmium in a carbon matrix, it was found that for 1-microm-thick samples the bright-field (BF) and annular dark-field (ADF) signals give similar contrast and signal-to-noise ratio provided the ADF inner angle and BF outer angle are chosen optimally. Spatial resolution in STEM imaging of thick sections is compromised by multiple elastic scattering which results in a spread of scattering angles and thus a spread in lateral distances of the electrons leaving the bottom surface. However, the simulations reveal that a large fraction of these multiply scattered electrons are excluded from the BF detector, which results in higher spatial resolution in BF than in high-angle ADF images for objects situated towards the bottom of the sample. The calculations imply that STEM electron tomography of thick sections should be performed using a BF rather than an ADF detector. This advantage was verified by recording simultaneous BF and high-angle ADF STEM tomographic tilt series from a stained 600-nm-thick section of C. elegans. It was found that loss of spatial resolution occurred markedly at the bottom surface of the specimen in the ADF STEM but significantly less in the BF STEM tomographic reconstruction. Our results indicate that it might be feasible to use BF STEM tomography to determine the 3D structure of whole eukaryotic microorganisms prepared by freeze-substitution, embedding, and sectioning.
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