Evidence map›Paper›PMID 18359249›Full record

ArticleJournal of structural biology2008

Determination of quantitative distributions of heavy-metal stain in biological specimens by annular dark-field STEM.

A A Sousa, M Hohmann-Marriott, M A Aronova, G Zhang, R D Leapman

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of structural biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
5.0field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 32 citations in OpenAlex.

  1. Article
  2. Article
  3. Detection of isolated protein-bound metal ions by single-particle cryo-STEM.Proceedings of the National Academy of Sciences of the United States of America · 2017
    Article
  4. Article
  5. Stomatobaculum longum gen. nov., sp. nov., an obligately anaerobic bacterium from the human oral cavity.International journal of systematic and evolutionary microbiology · 2013
    Article
  6. Review
  7. Article
  8. The probe profile and lateral resolution of scanning transmission electron microscopy of thick specimens.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2012
    Article
  9. Article
  10. Simulating STEM imaging of nanoparticles in micrometers-thick substrates.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2010
    Article
  11. Article
  12. Three-dimensional scanning transmission electron microscopy of biological specimens.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2010
    Article
  13. Article
  14. Article
  15. Electron microscopy of whole cells in liquid with nanometer resolution.Proceedings of the National Academy of Sciences of the United States of America · 2009
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

A A SousaLaboratory of Bioengineering and Physical Science, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Building 13, Room 3N17, 13 South Drive, Bethesda, MD 20892, USA.
M Hohmann-Marriott
M A Aronova
G Zhang
R D Leapman
National Institutes of Health · USNational Institute of Biomedical Imaging and Bioengineering · US

Funding

Scanning Transmission Electron Tomography of Biological StructuresZ01EB000046 · NIBIB · NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING · PI LEAPMAN, RICHARD · 2007 to 2008
$95k
Intramural NIH HHS Z01 EB000046
6 · The paper itself

Abstract

It is shown that dark-field images collected in the scanning transmission electron microscope (STEM) at two different camera lengths yield quantitative distributions of both the heavy and light atoms in a stained biological specimen. Quantitative analysis of the paired STEM images requires knowledge of the elastic scattering cross sections, which are calculated from the NIST elastic scattering cross section database. The results reveal quantitative information about the distribution of fixative and stain within the biological matrix, and provide a basis for assessing detection limits for heavy-metal clusters used to label intracellular proteins. In sectioned cells that have been stained only with osmium tetroxide, we find an average of 1.2+/-0.1 Os atom per nm(3), corresponding to an atomic ratio of Os:C atoms of approximately 0.02, which indicates that small heavy atom clusters of Undecagold and Nanogold can be detected in lightly stained specimens.

Indexed as

ChlorophytaImmunohistochemistryMetals, HeavyMicroscopy, Electron, Scanning TransmissionReproducibility of ResultsThylakoidsMetals, Heavy

Identifiers

PMID18359249
PMCPMC2705981
OpenAlexW2016798351

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.