ArticleMolecular and cellular biology2007
Regulation of the Il4 gene is independently controlled by proximal and distal 3' enhancers in mast cells and basophils.
Article in Molecular and cellular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 30 citations in OpenAlex.
- Diverse innate stimuli activate basophils through pathways involving Syk and IκB kinases.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Characterization of Cytokines and Proliferation Marker Ki67 in Cleft Affected Lip Tissue.Medicina (Kaunas, Lithuania) · 2019Article
- Stabilization of cytokine mRNAs in iNKT cells requires the serine-threonine kinase IRE1alpha.Nature communications · 2018Article
- Article
- Approaches for analyzing the roles of mast cells and their proteases in vivo.Advances in immunology · 2015Review
- Lymphocyte-based model systems for allergy research: a historic overview.International archives of allergy and immunology · 2014Review
- GATA-1 regulates the generation and function of basophils.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Differentiation of innate type-2 effector cells.Immunologic research · 2013Review
- Targeted deletion of the antisilencer/enhancer (ASE) element from intron 1 of the myelin proteolipid protein gene (Plp1) in mouse reveals that the element is dispensable for Plp1 expression in brain during development and remyelination.Journal of neurochemistry · 2013Article
- Basophils are required for the induction of Th2 immunity to haptens and peptide antigens.Nature communications · 2013Article
- Review
- New models for analyzing mast cell functions in vivo.Trends in immunology · 2012Review
- Interleukin-4 production by follicular helper T cells requires the conserved Il4 enhancer hypersensitivity site V.Immunity · 2012Article
- p53 negatively regulates RGS13 protein expression in immune cells.The Journal of biological chemistry · 2011Article
- CCAAT/enhancer-binding protein alpha (C/EBPalpha) is critical for interleukin-4 expression in response to FcepsilonRI receptor cross-linking.The Journal of biological chemistry · 2011Article
- The enhancer HS2 critically regulates GATA-3-mediated Il4 transcription in T(H)2 cells.Nature immunology · 2011Article
- Requirement of interaction between mast cells and skin dendritic cells to establish contact hypersensitivity.PloS one · 2011Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mast cells and basophils are known to be a critical interleukin 4 (IL-4) source for establishing Th2 protective responses to parasitic infections. Chromatin structure and histone modification patterns in the Il13/Il4 locus of mast cells were similar to those of IL-4-producing type 2 helper T cells. However, using a transgenic approach, we found that Il4 gene expression was distinctly regulated by individual cis regulatory elements in cell types of different lineages. The distal 3' element contained conserved noncoding sequence 2 (CNS-2), which was a common enhancer for memory phenotype T cells, NKT cells, mast cells, and basophils. Targeted deletion of CNS-2 compromised production of IL-4 and several Th2 cytokines in connective-tissue-type and immature-type mast cells but not in basophils. Interestingly, the proximal 3' element containing DNase I-hypersensitive site 4 (HS4), which controls Il4 gene silencing in T-lineage cells, exhibited selective enhancer activity in basophils. These results indicate that CNS-2 is an essential enhancer for Il4 gene transcription in mast cell but not in basophils. The transcription of the Il4 gene in mast cells and basophils is independently regulated by CNS-2 and HS4 elements that may be critical for lineage-specific Il4 gene regulation in these cell types.
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