ArticleJournal of molecular histology2008
Microwave oven-based technique for immunofluorescent staining of paraffin-embedded tissues.
Article in Journal of molecular histology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 31 citations in OpenAlex.
- Microwave-assisted immunostaining for rapid labeling of matrix-embedded multicellular structures.APL bioengineering · 2025Article
- Accelerated protein retention expansion microscopy using microwave radiation.Cell reports methods · 2024Article
- Sox9-Meis1 Inactivation Is Required for Adipogenesis, Advancing Pref-1Cell reports · 2018Article
- Clearing and Labeling Techniques for Large-Scale Biological Tissues.Molecules and cells · 2016Review
- Eliminating Size-Associated Diffusion Constraints for Rapid On-Surface Bioassays with Nanoparticle Probes.Small (Weinheim an der Bergstrasse, Germany) · 2016Article
- Microwave-Assisted Tissue Preparation for Rapid Fixation, Decalcification, Antigen Retrieval, Cryosectioning, and Immunostaining.International journal of cell biology · 2016Review
- Novel surface markers directed against adult human gallbladder.Stem cell research · 2015Article
- MicroRNA-146b-3p regulates retinal inflammation by suppressing adenosine deaminase-2 in diabetes.BioMed research international · 2015Article
- The regulation of reproductive neuroendocrine function by insulin and insulin-like growth factor-1 (IGF-1).Frontiers in neuroendocrinology · 2014Review
- Article
- Presence of interleukin-17C in the tissue around aseptic loosened implants.International orthopaedics · 2013Article
- Antigen retrieval immunohistochemistry: review and future prospects in research and diagnosis over two decades.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2011Review
- A guide to the perplexed on the specificity of antibodies.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2009Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Immunohistochemical analysis of formalin-fixed paraffin-embedded tissues can be challenging due to potential modifications of protein structure by exposure to formalin. Heat-induced antigen retrieval techniques can reverse reactions between formalin and proteins that block antibody recognition. Interactions between antibodies and antigens are further enhanced by microwave irradiation, which has simplified immunohistochemical staining protocols. In this report, we modify a technique for antigen retrieval and immunofluorescent staining of formalin-fixed paraffin-embedded tissues by showing that it works well with several antibodies and buffers. This microwave-assisted method for antigen retrieval and immunofluorescent staining eliminates the need for blocking reagents and extended washes, which greatly simplifies the protocol allowing one to complete the analysis in less than 3 h.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.