ArticlePlant physiology2004
Protein cryoprotective activity of a cytosolic small heat shock protein that accumulates constitutively in chestnut stems and is up-regulated by low and high temperatures.
Article in Plant physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 97 citations in OpenAlex.
- Heat Shock Proteins in Medically Relevant Fungal Pathogens: From Molecular Chaperones to Virulence Factors and Therapeutic Targets.Journal of fungi (Basel, Switzerland) · 2026Review
- HSP104 and HSP20-L Are Required by Aspergillus nidulans in Response to Attack by Fungivorous Springtail Sinella curviseta.Environmental microbiology reports · 2025Article
- Genome-Wide Identification and Evolution-Profiling Analysis ofInternational journal of molecular sciences · 2025Article
- Fungal heat shock proteins: molecular phylogenetic insights into the host takeover.Die Naturwissenschaften · 2024Article
- Exogenous application of salicylic acid improves freezing stress tolerance in alfalfa.Frontiers in plant science · 2023Article
- Genome-wide transcriptional changes triggered by water deficit on a drought-tolerant common bean cultivar.BMC plant biology · 2020Article
- Exogenous Ascorbic Acid Induced Chilling Tolerance in Tomato Plants Through Modulating Metabolism, Osmolytes, Antioxidants, and Transcriptional Regulation of Catalase and Heat Shock Proteins.Plants (Basel, Switzerland) · 2020Article
- Changes Induced by Pressure Processing on Immunoreactive Proteins of Tree Nuts.Molecules (Basel, Switzerland) · 2020Article
- A class I cytosolic HSP20 of rice enhances heat and salt tolerance in different organisms.Scientific reports · 2020Article
- The Mitochondrial Small Heat Shock Protein HSP22 from Pea is a Thermosoluble Chaperone Prone to Co-Precipitate with Unfolding Client Proteins.International journal of molecular sciences · 2019Article
- Genome-wide analysis and expression profiling under heat and drought treatments of HSP70 gene family in soybean (Glycine max L.).Frontiers in plant science · 2015Article
- Combined Treatments Reduce Chilling Injury and Maintain Fruit Quality in Avocado Fruit during Cold Quarantine.PloS one · 2015Article
- Role of Heat-Shock Proteins in Cellular Function and in the Biology of Fungi.Biotechnology research international · 2015Review
- Plantation forestry under global warming: hybrid poplars with improved thermotolerance provide new insights on the in vivo function of small heat shock protein chaperones.Plant physiology · 2014Article
- Expression of selected Ginkgo biloba heat shock protein genes after cold treatment could be induced by other abiotic stress.International journal of molecular sciences · 2012Article
- Developmental transcriptomic features of the carcinogenic liver fluke, Clonorchis sinensis.PLoS neglected tropical diseases · 2011Article
- Cryoprotective mechanism of a small intrinsically disordered dehydrin protein.Protein science : a publication of the Protein Society · 2011Article
- Involvement of CBF transcription factors in winter hardiness in birch.Plant physiology · 2008Article
- Molecular chaperone activity of tomato (Lycopersicon esculentum) endoplasmic reticulum-located small heat shock protein.Journal of plant research · 2008Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Heat shock, and other stresses that cause protein misfolding and aggregation, trigger the accumulation of heat shock proteins (HSPs) in virtually all organisms. Among the HSPs of higher plants, those belonging to the small HSP (sHSP) family remain the least characterized in functional terms. We analyzed the occurrence of sHSPs in vegetative organs of Castanea sativa (sweet chestnut), a temperate woody species that exhibits remarkable freezing tolerance. A constitutive sHSP subject to seasonal periodic changes of abundance was immunodetected in stems. This protein was identified by matrix-assisted laser-desorption ionization time of flight mass spectrometry and internal peptide sequencing as CsHSP17.5, a cytosolic class I sHSP previously described in cotyledons. Expression of the corresponding gene in stems was confirmed through cDNA cloning and reverse transcription-PCR. Stem protein and mRNA profiles indicated that CsHSP17.5 is significantly up-regulated in spring and fall, reaching maximal levels in late summer and, especially, in winter. In addition, cold exposure was found to quickly activate shsp gene expression in both stems and roots of chestnut seedlings kept in growth chambers. Our main finding is that purified CsHSP17.5 is very effective in protecting the cold-labile enzyme lactate dehydrogenase from freeze-induced inactivation (on a molar basis, CsHSP17.5 is about 400 times more effective as cryoprotectant than hen egg-white lysozyme). Consistent with these observations, repeated freezing/thawing did not affect appreciably the chaperone activity of diluted CsHSP17.5 nor its ability to form dodecameric complexes in vitro. Taken together, these results substantiate the hypothesis that sHSPs can play relevant roles in the acquisition of freezing tolerance.
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