ArticleJournal of applied physiology (Bethesda, Md. : 1985)2002
Biopsy coupled to quantitative immunofluorescence: a new method to study the human vascular endothelium.
Article in Journal of applied physiology (Bethesda, Md. : 1985), 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT05478707 (Therapeutic Strategies for Microvascular Dysfunction in Type 1 Diabetes), which is not on this map. Cited by 53 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Therapeutic Strategies for Microvascular Dysfunction in Type 1 Diabetes
Who cites it
53 citing papers in PubMed, 79 citations in OpenAlex.
- Differential effects of nebivolol vs. metoprolol on microvascular function in hypertensive humans.American journal of physiology. Heart and circulatory physiology · 2016Trial
- Acute effect of mineralocorticoid receptor antagonism on vascular function in healthy older adults.Experimental gerontology · 2016Trial
- A novel minimally-invasive method to sample human endothelial cells for molecular profiling.PloS one · 2015Trial
- Mineralocorticoid receptors modulate vascular endothelial function in human obesity.Clinical science (London, England : 1979) · 2013Trial
- Nuclear factor-{kappa}B activation contributes to vascular endothelial dysfunction via oxidative stress in overweight/obese middle-aged and older humans.Circulation · 2009Trial
- Xanthine oxidase does not contribute to impaired peripheral conduit artery endothelium-dependent dilatation with ageing.The Journal of physiology · 2006Trial
- Iron sucrose augments homocysteine-induced endothelial dysfunction in normal subjects.Kidney international · 2006Trial
- Endothelial Hydrogels Improve Microvascular Regeneration and Perfusion in Tracheal Scaffolds.The Laryngoscope · 2026Article
- Endothelial dysfunction in middle-aged and older men with low testosterone is associated with elevated circulating endothelin-1.American journal of physiology. Regulatory, integrative and comparative physiology · 2025Article
- Effects of circulating endothelial microvesicles isolated from adults with obesity on endothelial cell inflammation, apoptosis, and nitric oxide production.American journal of physiology. Endocrinology and metabolism · 2024Article
- Association between atherogenic risk-modulating proteins and endothelium-dependent flow-mediated dilation in coronary artery disease patients.European journal of applied physiology · 2023Article
- Generation of a Novel In Vitro Model to Study Endothelial Dysfunction from Atherothrombotic Specimens.Cardiovascular drugs and therapy · 2021Article
- Impact of catheterization on shear-mediated arterial dilation in healthy young men.European journal of applied physiology · 2020Article
- Vascular Endothelial Function in Midlife/Older Adults Classified According to 2017 American College of Cardiology/American Heart Association Blood Pressure Guidelines.Journal of the American Heart Association · 2020Article
- Altered endothelial ETAmerican journal of physiology. Heart and circulatory physiology · 2020Article
- Trimethylamine-N-Oxide Promotes Age-Related Vascular Oxidative Stress and Endothelial Dysfunction in Mice and Healthy Humans.Hypertension (Dallas, Tex. : 1979) · 2020Article
- Alterations in Vascular Function Associated With the Use of Combustible and Electronic Cigarettes.Journal of the American Heart Association · 2020Article
- Overview of the Assessment of Endothelial Function in Humans.Frontiers in medicine · 2020Review
- Comparative Transcriptomics ofJACC. Basic to translational science · 2019Article
- Generation of Endothelial Cells From Human Pluripotent Stem Cells.Arteriosclerosis, thrombosis, and vascular biology · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 1 institution in 1 country.
Funding
Abstract
Limited availability of endothelial tissue is a major constraint when investigating the cellular mechanisms of endothelial dysfunction in patients with metabolic and cardiovascular diseases. We propose a novel approach that combines collection of 200-1,000 endothelial cells from a superficial forearm vein or the radial artery, with reliable measurements of protein expression by quantitative immunofluorescence analysis. This method was validated against immunoblot analysis in cultured endothelial cells. Levels of vascular endothelial cell activation, oxidative stress, and nitric oxide synthase expression were measured and compared in five patients with severe chronic heart failure and in four healthy age-matched subjects. In summary, vascular endothelial biopsy coupled with measurement of protein expression by quantitative immunofluorescence analysis provides a novel approach to the study of the vascular endothelium in humans.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.