ArticleProceedings of the National Academy of Sciences of the United States of America2001
An isolated, surface-expressed I domain of the integrin alphaLbeta2 is sufficient for strong adhesive function when locked in the open conformation with a disulfide bond.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 126 citations in OpenAlex.
- Review
- Humanized β2 Integrin-Expressing Hoxb8 Cells Serve as Model to Study Integrin Activation.Cells · 2022Article
- Context-Dependent Role of Vinculin in Neutrophil Adhesion, Motility and Trafficking.Scientific reports · 2020Article
- Ligand-specific binding forces of LFA-1 and Mac-1 in neutrophil adhesion and crawling.Molecular biology of the cell · 2018Article
- Chemistry and Enzymology of Disulfide Cross-Linking in Proteins.Chemical reviews · 2018Review
- LFA-1 in T Cell Migration and Differentiation.Frontiers in immunology · 2018Review
- Dramatic and concerted conformational changes enable rhodocetin to block α2β1 integrin selectively.PLoS biology · 2017Article
- Activation mechanisms of αVβ3 integrin by binding to fibronectin: A computational study.Protein science : a publication of the Protein Society · 2017Article
- Article
- Structure and dynamics of the integrin LFA-1 I-domain in the inactive state underlie its inside-out/outside-in signaling and allosteric mechanisms.Structure (London, England : 1993) · 2015Article
- Ligation of ICAM-1 on human aortic valve interstitial cells induces the osteogenic response: A critical role of the Notch1-NF-κB pathway in BMP-2 expression.Biochimica et biophysica acta · 2014Article
- Adhesive dynamics.Journal of biomechanical engineering · 2014Review
- An integrin binding-defective mutant of insulin-like growth factor-1 (R36E/R37E IGF1) acts as a dominant-negative antagonist of the IGF1 receptor (IGF1R) and suppresses tumorigenesis but still binds to IGF1R.The Journal of biological chemistry · 2013Article
- Structure of Streptococcus agalactiae tip pilin GBS104: a model for GBS pili assembly and host interactions.Acta crystallographica. Section D, Biological crystallography · 2013Article
- Isoflurane binds and stabilizes a closed conformation of the leukocyte function-associated antigen-1.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2012Article
- Reconstruction of integrin activation.Blood · 2012Review
- Neutrophil arrest by LFA-1 activation.Frontiers in immunology · 2012Article
- The C-terminal αI domain linker as a critical structural element in the conformational activation of αI integrins.The Journal of biological chemistry · 2011Article
- Regulation of integrin affinity on cell surfaces.The EMBO journal · 2011Article
- Sedative drug modulates T-cell and lymphocyte function-associated antigen-1 function.Anesthesia and analgesia · 2011Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
We introduced disulfide bonds to lock the integrin alphaLbeta2 I domain in predicted open, ligand binding or closed, nonbinding conformations. Transfectants expressing alphaLbeta2 heterodimers containing locked-open but not locked-closed or wild-type I domains constitutively adhered to intercellular adhesion molecule-1 (ICAM-1) substrates. Locking the I domain closed abolished constitutive and activatable adhesion. The isolated locked-open I domain bound as well as the activated alphaLbeta2 heterodimer, and binding was abolished by reduction of the disulfide. Lovastatin, which binds under the conformationally mobile C-terminal alpha-helix of the I domain, inhibited binding to ICAM-1 by alphaLbeta2 with wild-type, but not locked-open I domains. These data establish the importance of conformational change in the alphaL I domain for adhesive function and show that this domain is sufficient for full adhesive activity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.