ArticleProceedings of the National Academy of Sciences of the United States of America1999
Recombination and transcription of the endogenous Ig heavy chain locus is effected by the Ig heavy chain intronic enhancer core region in the absence of the matrix attachment regions.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 98 citations in OpenAlex.
- Lineage- and stage-specific activity of antigen receptor gene enhancers during lymphocyte development.Frontiers in epigenetics and epigenomics · 2024Review
- A dual function for the chromatin organizer Special A-T rich Binding Protein 1 in B-lineage cells.Cellular & molecular immunology · 2023Article
- The IgHFrontiers in immunology · 2023Article
- Long-Range Control of Class Switch Recombination by Transcriptional Regulatory Elements.Frontiers in immunology · 2021Review
- HMGA Proteins in Hematological Malignancies.Cancers · 2020Review
- Sequential activation and distinct functions for distal and proximal modules within the IgH 3' regulatory region.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Allelic exclusion of the immunoglobulin heavy chain locus is independent of its nuclear localization in mature B cells.Nucleic acids research · 2013Article
- The IgH 3' regulatory region controls somatic hypermutation in germinal center B cells.The Journal of experimental medicine · 2013Article
- Germline deletion of Igh 3' regulatory region elements hs 5, 6, 7 (hs5-7) affects B cell-specific regulation, rearrangement, and insulation of the Igh locus.Journal of immunology (Baltimore, Md. : 1950) · 2012Article
- Two forms of loops generate the chromatin conformation of the immunoglobulin heavy-chain gene locus.Cell · 2011Article
- Article
- Elements between the IgH variable (V) and diversity (D) clusters influence antisense transcription and lineage-specific V(D)J recombination.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Article
- Switch region identity plays an important role in Ig class switch recombination.Journal of immunology (Baltimore, Md. : 1950) · 2010Article
- SATB1 binds an intronic MAR sequence in human PI3kgamma in vitro.Molecular biology reports · 2010Article
- A 220-nucleotide deletion of the intronic enhancer reveals an epigenetic hierarchy in immunoglobulin heavy chain locus activation.The Journal of experimental medicine · 2009Article
- Mi2beta shows chromatin enzyme specificity by erasing a DNase I-hypersensitive site established by ACF.The Journal of biological chemistry · 2009Article
- A role for the IgH intronic enhancer E mu in enforcing allelic exclusion.The Journal of experimental medicine · 2009Article
- Requirement for enhancer specificity in immunoglobulin heavy chain locus regulation.Journal of immunology (Baltimore, Md. : 1950) · 2008Article
- Cis-regulatory elements and epigenetic changes control genomic rearrangements of the IgH locus.Advances in immunology · 2008Review
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The intronic Ig heavy chain (IgH) enhancer, which consists of the core enhancer flanked by 5' and 3' matrix attachment regions, has been implicated in control of IgH locus recombination and transcription. To elucidate the regulatory functions of the core enhancer and its associated matrix attachment regions in the endogenous IgH locus, we have introduced targeted deletions of these elements, both individually and in combination, into an IgHa/b-heterozygous embryonic stem cell line. These embryonic stem cells were used to generate chimeric mice by recombination activating gene-2 (Rag-2)-deficient blastocyst complementation, and the effects of the introduced mutations were assayed in mutant B cells. We find that the core enhancer is necessary and sufficient to promote normal variable (V), diversity (D), and joining (J) segment recombination in developing B lineage cells and IgH locus transcription in mature B cells. Surprisingly, the 5' and 3' matrix attachment regions were dispensable for these processes.
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