ArticleJournal of clinical microbiology1999
PCR detection of DNA specific for Trichosporon species in serum of patients with disseminated trichosporonosis.
Article in Journal of clinical microbiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 44 citations in OpenAlex.
- BeyondJournal of fungi (Basel, Switzerland) · 2026Article
- InvasiveJournal of fungi (Basel, Switzerland) · 2025Article
- A Rare Case of a Retroperitoneal Abscess Due to Trichosporon spp. in an Immunocompetent Patient.Cureus · 2024Article
- Strain Typing ofJournal of laboratory physicians · 2021Article
- Molecular Diagnosis of Yeast Infections.Current fungal infection reports · 2021Review
- Subcutaneous Infection Associated with Trichosporon ovoides: A Case Report and Review of Literature.Mycopathologia · 2016Review
- The first isolation ofCurrent medical mycology · 2015Article
- Development of a peptide nucleic acid probe to Trichosporon species and identification of trichosporonosis by use of in situ hybridization in formalin-fixed and paraffin-embedded (FFPE) sections.Journal of clinical microbiology · 2013Article
- Current knowledge of Trichosporon spp. and Trichosporonosis.Clinical microbiology reviews · 2011Review
- Trichosporon species isolated from the perigenital region, urine and catheters of a brazilian population.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2010Article
- Genetic diversity and antifungal susceptibility testing of Trichosporon asahii isolated of Intensive Care Units patients.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2008Article
- Supplemental utility of nested PCR for the pathological diagnosis of disseminated trichosporonosis.Virchows Archiv : an international journal of pathology · 2007Article
- High-throughput detection of pathogenic yeasts of the genus trichosporon.Journal of clinical microbiology · 2004Article
- Edible dates (Phoenix dactylifera), a potential source of Cladosporium cladosporioides and Sporobolomyces roseus: implications for public health.Mycopathologia · 2002Article
- Rapid identification of yeasts in positive blood cultures by a multiplex PCR method.Journal of clinical microbiology · 2001Article
- Experimental model of progressive disseminated trichosporonosis in mice with latent trichosporonemia.Journal of clinical microbiology · 2000Article
- Reverse cross blot hybridization assay for rapid detection of PCR-amplified DNA from candida species, Cryptococcus neoformans, and Saccharomyces cerevisiae in clinical samples.Journal of clinical microbiology · 2000Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Deep-seated trichosporonosis is a lethal opportunistic infection that disseminates rapidly and widely in immunocompromised patients, and early diagnosis is crucial for the treatment of this infection. We developed a novel nested-PCR assay that detects DNA specific for clinically important strains of Trichosporon in serum samples from patients with disseminated trichosporonosis. In this assay, two sets of oligonucleotide primers were derived from the sequence of 26S rRNA genes of Trichosporon asahii. The specific fragment was amplified from T. asahii and T. mucoides, but not from other microorganisms, including some other basidiomycetous fungi (Cryptococcus, Malassezia, Rhodotorula, and Sporobolomyces). Target DNA was detected by the nested PCR with as little as 5 fg of the extracted DNA of T. asahii. In a study using 11 clinical samples, the specific fragment was detected by the nested PCR in 64% (7 of 11) of sera from patients with histologically diagnosed disseminated trichosporonosis, while glucuronoxylomannan antigen was detected in only 54% (6 of 11) of the samples. Our new nested-PCR assay using serum samples can be performed repeatedly throughout the course of the disease. In addition, not only can it be used for early diagnosis of trichosporonosis, but it may also be beneficial for monitoring its progress or response to therapy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.