ArticleJournal of clinical microbiology1999
Rapid identification and typing of Staphylococcus aureus by PCR-restriction fragment length polymorphism analysis of the aroA gene.
Article in Journal of clinical microbiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Exploration of Antibiotic Activity of Aminoglycosides, in Particular Ribostamycin Alone and in Combination With Ethylenediaminetetraacetic Acid Against Pathogenic Bacteria.Frontiers in microbiology · 2020Article
- Methicillin resistance and selective genetic determinants ofIranian journal of microbiology · 2017Article
- Limit of detection of genomic DNA by conventional PCR for estimating the load of Staphylococcus aureus and Escherichia coli associated with bovine mastitis.Folia microbiologica · 2015Article
- Detection of the enterotoxigenic genes (Comparative clinical pathology · 2013Article
- Use of 5-Enolpyruvylshikmate-3-Phosphate Synthase Encoding Gene for Typing of Staphylococcus aureus Isolated from Skin and Urinary Tract Infections of Human.Iranian journal of basic medical sciences · 2012Article
- High-throughput molecular identification of Staphylococcus spp. isolated from a clean room facility in an environmental monitoring program.BMC research notes · 2010Article
- Molecular diagnostics of clinically important staphylococci.Folia microbiologica · 2004Review
- Identification of Staphylococcus spp. by PCR-restriction fragment length polymorphism of gap gene.Journal of clinical microbiology · 2001Article
- Glyceraldehyde-3-phosphate dehydrogenase-encoding gene as a useful taxonomic tool for Staphylococcus spp.Journal of clinical microbiology · 2000Article
- Molecular cloning and sequencing of the aroA gene from Actinobacillus pleuropneumoniae and its use in a PCR assay for rapid identification.Journal of clinical microbiology · 1999Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Staphylococcus aureus aroA gene, which encodes 5-enolpyruvylshikimate-3-phosphate synthase, was used as a target for the amplification of a 1,153-bp DNA fragment by PCR with a pair of primers of 24 and 19 nucleotides. The PCR products, which were detected by agarose gel electrophoresis, were amplified from all S. aureus strains so far analyzed (reference strains and isolates from cows and sheep with mastitis, as well as 59 isolates from humans involved in four confirmed outbreaks). Hybridization with an internal 536-bp DNA fragment probe was positive for all PCR-positive samples. No PCR products were amplified when other Staphylococcus spp. or genera were analyzed by using the same pair of primers. The detection limit for S. aureus cells was 20 CFU when the cells were suspended in saline; however, the sensitivity of the PCR was lower (5 x 10(2) CFU) when S. aureus cells were suspended in sterilized whole milk. TaqI digestion of the PCR-generated products rendered two different restriction fragment length polymorphism patterns with the cow and sheep strains tested, and these patterns corresponded to the two different patterns obtained by antibiotic susceptibility tests. Analysis of the 59 human isolates by our easy and rapid protocol rendered results similar to those of other assays.
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